LOCUS V038580 6942 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038580 VERSION V038580 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 927..1745 /label="PAQR4(NM_152341)" /note="PAQR4(NM_152341)" /gene="PAQR4" CDS 1785..2504 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2542..2766 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2812..3240 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3254..3583 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3650..4444 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4618..4739 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4788..4804) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4812..4828 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4836..4866) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4881..4902 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5190..5775) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5946..6806) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6807..6911) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc gccaccatgg cgttcctggc cgggccgcgc ctgctggact 961 gggccagctc gccgccgcac ctgcagttca ataagttcgt gctgaccggg taccggcccg 1021 ccagcagcgg ctcgggctgc ctgcgcagcc tcttctacct gcacaacgaa ctgggcaaca 1081 tctacacgca cgggctggcc ctgctgggct tcctggtgct ggtgccaatg accatgccct 1141 ggggtcagct gggcaaggat ggctggctgg gaggcacaca ttgcgtggcc tgccttgcac 1201 cccctgcagg ctccgtgctc tatcacctct ttatgtgcca ccaagggggc agcgctgtgt 1261 acgcccggct cctcgccctg gacatgtgtg gggtctgcct tgtcaacacc cttggggccc 1321 tgcccatcat ccactgcacc ctggcctgca ggccctggct gcgcccggct gccctggtgg 1381 gctacactgt gttgtcgggt gtggccggct ggcgtgctct caccgccccc tccaccagtg 1441 ctcggctccg ggcatttgga tggcaggctg ctgcccgcct actggtattt ggggcccggg 1501 gagtgggtct gggttcaggg gctccaggct ccctgccctg ctacctgcgc atggacgcac 1561 tggcgctgct tgggggactg gtaaatgtag cccgtctgcc cgagcgctgg ggacctggcc 1621 gctttgacta ctggggcaac tcccaccaga tcatgcacct gctgagcgtg ggctccatcc 1681 tgcagctgca cgccggcgtc gtgcccgacc tgctctgggc tgcccaccac gcctgtcccc 1741 gggacacctc gagtggcggt ggaggaagcg gcggaggtgg atctatggtg agcaagggcg 1801 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 1861 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 1921 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 1981 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 2041 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 2101 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 2161 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 2221 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 2281 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 2341 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 2401 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 2461 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtagtctaga gggcccgttt 2521 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2701 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 2761 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 2821 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 2881 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 2941 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 3001 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3061 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3121 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3181 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3241 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3301 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3421 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3481 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3541 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3601 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3661 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3721 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3781 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 3841 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3901 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3961 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 4021 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4081 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4141 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4201 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4261 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4321 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4381 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4441 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4501 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4561 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 4621 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4681 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 4741 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 4801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 4861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 4921 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 4981 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 5041 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5101 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 5161 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5221 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 5281 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 5341 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 5401 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 5461 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 5521 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 5581 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 5641 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 5701 ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 5761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5881 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5941 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 6001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6901 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //