LOCUS       pcDNA3.1-PKD2(human)    8324 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029444
VERSION     V029444
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3811
                     /label="PKD2(NM_000297)"
                     /note="PKD2(NM_000297)"
                     /gene="PKD2"
     polyA_signal    3891..4115
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4161..4589
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4603..4932
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4999..5793
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5967..6088
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6137..6153)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6161..6177
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6185..6215)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6230..6251
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6539..7124)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7295..8155)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8156..8260)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gtgaactcca gtcgcgtgca gcctcagcag cccggggacg ccaagcggcc
      961 gcccgcgccc cgcgcgccgg acccgggccg gctgatggct ggctgcgcgg ccgtgggcgc
     1021 cagcctcgcc gccccgggcg gcctctgcga gcagcggggc ctggagatcg agatgcagcg
     1081 catccggcag gcggccgcgc gggacccccc ggccggagcc gcggcctccc cttctcctcc
     1141 gctctcgtcg tgctcccggc aggcgtggag ccgcgataac cccggcttcg aggccgagga
     1201 ggaggaggag gaggtggaag gggaagaagg cggaatggtg gtggagatgg acgtagagtg
     1261 gcgcccgggc agccggaggt cggccgcctc ctcggccgtg agctccgtgg gcgcgcggag
     1321 ccgggggctt gggggctacc acggcgcggg ccacccgagc gggaggcggc gccggcgaga
     1381 ggaccagggc ccgccgtgcc ccagcccagt cggcggcggg gacccgctgc atcgccacct
     1441 ccccctggaa gggcagccgc cccgagtggc ctgggcggag aggctggttc gcgggctgcg
     1501 aggtctctgg ggaacaagac tcatggagga aagcagcact aaccgagaga aataccttaa
     1561 aagtgtttta cgggaactgg tcacatacct cctttttctc atagtcttgt gcatcttgac
     1621 ctacggcatg atgagctcca atgtgtacta ctacacccgg atgatgtcac agctcttcct
     1681 agacaccccc gtgtccaaaa cggagaaaac taactttaaa actctgtctt ccatggaaga
     1741 cttctggaag ttcacagaag gctccttatt ggatgggctg tactggaaga tgcagcccag
     1801 caaccagact gaagctgaca accgaagttt catcttctat gagaacctgc tgttaggggt
     1861 tccacgaata cggcaactcc gagtcagaaa tggatcctgc tctatccccc aggacttgag
     1921 agatgaaatt aaagagtgct atgatgtcta ctctgtcagt agtgaagata gggctccctt
     1981 tgggccccga aatggaaccg cttggatcta cacaagtgaa aaagacttga atggtagtag
     2041 ccactgggga atcattgcaa cttatagtgg agctggctat tatctggatt tgtcaagaac
     2101 aagagaggaa acagctgcac aagttgctag cctcaagaaa aatgtctggc tggaccgagg
     2161 aaccagggca acttttattg acttctcagt gtacaacgcc aacattaacc tgttctgtgt
     2221 ggtcaggtta ttggttgaat tcccagcaac aggtggtgtg attccatctt ggcaatttca
     2281 gcctttaaag ctgatccgat atgtcacaac ttttgatttc ttcctggcag cctgtgagat
     2341 tatcttttgt ttctttatct tttactatgt ggtggaagag atattggaaa ttcgcattca
     2401 caaactacac tatttcagga gtttctggaa ttgtctggat gttgtgatcg ttgtgctgtc
     2461 agtggtagct ataggaatta acatatacag aacatcaaat gtggaggtgc tactacagtt
     2521 tctggaagat caaaatactt tccccaactt tgagcatctg gcatattggc agatacagtt
     2581 caacaatata gctgctgtca cagtattttt tgtctggatt aagctcttca aattcatcaa
     2641 ttttaacagg accatgagcc agctctcgac aaccatgtct cgatgtgcca aagacctgtt
     2701 tggctttgct attatgttct tcattatttt cctagcgtat gctcagttgg cataccttgt
     2761 ctttggcact caggtcgatg acttcagtac tttccaagag tgtatcttca ctcaattccg
     2821 tatcattttg ggcgatatca actttgcaga gattgaggaa gctaatcgag ttttgggacc
     2881 aatttatttc actacatttg tgttctttat gttcttcatt cttttgaata tgtttttggc
     2941 tatcatcaat gatacttact ctgaagtgaa atctgacttg gcacagcaga aagctgaaat
     3001 ggaactctca gatcttatca gaaagggcta ccataaagct ttggtcaaac taaaactgaa
     3061 aaaaaatacc gtggatgaca tttcagagag tctgcggcaa ggaggaggca agttaaactt
     3121 tgacgaactt cgacaagatc tcaaagggaa gggccatact gatgcagaga ttgaggcaat
     3181 attcacaaag tacgaccaag atggagacca agaactgacc gaacatgaac atcagcagat
     3241 gagagacgac ttggagaaag agagggagga cctggatttg gatcacagtt ctttaccacg
     3301 tcccatgagc agccgaagtt tccctcgaag cctggatgac tctgaggagg atgacgatga
     3361 agatagcgga catagctcca gaaggagggg aagcatttct agtggcgttt cttacgaaga
     3421 gtttcaagtc ctggtgagac gagtggaccg gatggagcat tccatcggca gcatagtgtc
     3481 caagattgac gccgtgatcg tgaagctaga gattatggag cgagccaaac tgaagaggag
     3541 ggaggtgctg ggaaggctgt tggatggggt ggccgaggat gaaaggctgg gtcgtgacag
     3601 tgaaatccat agggaacaga tggaacggct agtacgtgaa gagttggaac gctgggaatc
     3661 cgatgatgca gcttcccaga tcagtcatgg tttaggcacg ccagtgggac taaatggtca
     3721 acctcgcccc agaagctccc gcccatcttc ctcccaatct acagaaggca tggaaggtgc
     3781 aggtggaaat gggagttcta atgtccacgt atgagaattc tgcagatatc cagcacagtg
     3841 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     3901 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3961 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4021 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     4081 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     4141 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4201 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4261 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4321 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4381 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4441 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4501 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4561 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     4621 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4681 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4741 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4801 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4861 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4921 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     4981 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     5041 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5101 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5161 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5221 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5281 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5341 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5401 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5461 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5521 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5581 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5641 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5701 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5761 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5821 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5881 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5941 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     6001 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6061 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     6121 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt
     7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8281 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggt
//
