LOCUS V038672 5953 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038672 VERSION V038672 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..1443 /label="PMP22(NM_153322)" /note="PMP22(NM_153322)" /gene="PMP22" CDS 1450..1515 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1553..1777 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1823..2251 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2265..2594 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2661..3455 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3629..3750 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3799..3815) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3823..3839 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3847..3877) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3892..3913 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4201..4786) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4957..5817) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5818..5922) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatgctcc tcctgttgct gagtatcatc gtcctccacg tcgcggtgct ggtgctgctg 1021 ttcgtctcca cgatcgtcag ccaatggatc gtgggcaatg gacacgcaac tgatctctgg 1081 cagaactgta gcacctcttc ctcaggaaat gtccaccact gtttctcatc atcaccaaac 1141 gaatggctgc agtctgtcca ggccaccatg atcctgtcga tcatcttcag cattctgtct 1201 ctgttcctgt tcttctgcca actcttcacc ctcaccaagg ggggcaggtt ttacatcact 1261 ggaatcttcc aaattcttgc tggtctgtgc gtgatgagtg ctgcggccat ctacacggtg 1321 aggcacccgg agtggcatct caactcggat tactcctacg gtttcgccta catcctggcc 1381 tgggtggcct tccccctggc ccttctcagc ggtgtcatct atgtgatctt gcggaaacgc 1441 gaactcgagg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag 1501 gatgacgatg acaagtagtg agggcccgtt taaacccgct gatcagcctc gactgtgcct 1561 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 1621 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 1681 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 1741 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 1801 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 1861 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 1921 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 1981 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 2041 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 2101 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 2161 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 2221 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 2281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2341 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2401 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2461 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2521 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2581 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 2641 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 2701 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 2761 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 2821 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 2881 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 2941 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 3001 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 3061 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 3121 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 3181 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 3241 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 3301 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 3361 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 3421 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 3481 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 3541 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 3601 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 3661 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 3721 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 3781 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 3841 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 3901 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 3961 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 4021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 4081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 4141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 4681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt 4741 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 4801 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 4861 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 4921 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 4981 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 5041 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 5101 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 5161 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 5221 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 5281 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 5341 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 5401 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 5461 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 5521 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 5581 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 5641 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 5701 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 5761 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 5821 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 5881 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 5941 gccacctgac gtc //