LOCUS V039318 6145 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039318 VERSION V039318 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..1635 /label="PRDX6(NM_004905)" /note="PRDX6(NM_004905)" /gene="PRDX6" CDS 1642..1707 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1745..1969 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2015..2443 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2457..2786 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2853..3647 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3821..3942 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3991..4007) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4015..4031 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4039..4069) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4084..4105 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4393..4978) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5149..6009) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6010..6114) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatgcccg gaggtctgct tctcggggac gtggctccca actttgaggc caataccacc 1021 gtcggccgca tccgtttcca cgactttctg ggagactcat ggggcattct cttctcccac 1081 cctcgggact ttaccccagt gtgcaccaca gagcttggca gagctgcaaa gctggcacca 1141 gaatttgcca agaggaatgt taagttgatt gccctttcaa tagacagtgt tgaggaccat 1201 cttgcctgga gcaaggatat caatgcttac aattgtgaag agcccacaga aaagttacct 1261 tttcccatca tcgatgatag gaatcgggag cttgccatcc tgttgggcat gctggatcca 1321 gcagagaagg atgaaaaggg catgcctgtg acagctcgtg tggtgtttgt ttttggtcct 1381 gataagaagc tgaagctgtc tatcctctac ccagctacca ctggcaggaa ctttgatgag 1441 attctcaggg tagtcatctc tctccagctg acagcagaaa aaagggttgc caccccagtt 1501 gattggaagg atggggatag tgtgatggtc cttccaacca tccctgaaga agaagccaaa 1561 aaacttttcc cgaaaggagt cttcaccaaa gagctcccat ctggcaagaa atacctccgc 1621 tacacacccc agcctctcga ggactacaaa gaccatgacg gtgattataa agatcatgac 1681 atcgactaca aggatgacga tgacaagtag tgagggcccg tttaaacccg ctgatcagcc 1741 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 1801 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 1861 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 1921 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 1981 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 2041 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 2101 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 2161 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 2221 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 2281 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 2341 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 2401 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 2461 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2521 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 2581 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 2641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 2701 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 2761 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 2821 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 2881 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 2941 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 3001 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 3061 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 3121 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 3181 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 3241 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 3301 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 3361 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 3421 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 3481 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 3541 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 3601 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 3661 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 3721 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 3781 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 3841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 3901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 3961 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 4021 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 4081 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 4141 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 4201 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 4261 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 4321 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 4381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 4441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 4501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 4561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 4621 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 4681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 4741 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 4801 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 4861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4921 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 5221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6121 tccccgaaaa gtgccacctg acgtc //