LOCUS       V022622                 7247 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022622
VERSION     V022622
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             902..2686
                     /label="PTPN6(NM_002831)"
                     /note="PTPN6(NM_002831)"
                     /gene="PTPN6"
     CDS             2693..2719
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2723..2749
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2753..2779
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2814..3038
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3084..3512
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3526..3855
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3922..4716
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4890..5011
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5060..5076)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5084..5100
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5108..5138)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5153..5174
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5462..6047)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6218..7078)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7079..7183)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcgccac
      901 catggtgagg tggtttcacc gagacctcag tgggctggat gcagagaccc tgctcaaggg
      961 ccgaggtgtc cacggtagct tcctggctcg gcccagtcgc aagaaccagg gtgacttctc
     1021 gctctccgtc agggtggggg atcaggtgac ccatattcgg atccagaact caggggattt
     1081 ctatgacctg tatggagggg agaagtttgc gactctgaca gagctggtgg agtactacac
     1141 tcagcagcag ggtgtcctgc aggaccgcga cggcaccatc atccacctca agtacccgct
     1201 gaactgctcc gatcccacta gtgagaggtg gtaccatggc cacatgtctg gcgggcaggc
     1261 agagacgctg ctgcaggcca agggcgagcc ctggacgttt cttgtgcgtg agagcctcag
     1321 ccagcctgga gacttcgtgc tttctgtgct cagtgaccag cccaaggctg gcccaggctc
     1381 cccgctcagg gtcacccaca tcaaggtcat gtgcgagggt ggacgctaca cagtgggtgg
     1441 tttggagacc ttcgacagcc tcacggacct ggtggagcat ttcaagaaga cggggattga
     1501 ggaggcctca ggcgcctttg tctacctgcg gcagccgtac tatgccacga gggtgaatgc
     1561 ggctgacatt gagaaccgag tgttggaact gaacaagaag caggagtccg aggatacagc
     1621 caaggctggc ttctgggagg agtttgagag tttgcagaag caggaggtga agaacttgca
     1681 ccagcgtctg gaagggcagc ggccagagaa caagggcaag aaccgctaca agaacattct
     1741 cccctttgac cacagccgag tgatcctgca gggacgggac agtaacatcc ccgggtccga
     1801 ctacatcaat gccaactaca tcaagaacca gctgctaggc cctgatgaga acgctaagac
     1861 ctacatcgcc agccagggtt gtctggaggc cacggtcaat gacttctggc agatggcgtg
     1921 gcaggagaac agccgtgtca tcgtcatgac cacccgagag gtggagaaag gccggaacaa
     1981 atgcgtccca tactggcccg aggtgggcat gcagcgtgct tatgggccct actctgtgac
     2041 caactgcggg gagcatgaca caaccgaata caaactccgt accttacagg tctccccgct
     2101 ggacaatgga gacctgattc gggagatctg gcattaccag tacctgagct ggcccgacca
     2161 tggggtcccc agtgagcctg ggggtgtcct cagcttcctg gaccagatca accagcggca
     2221 ggaaagtctg cctcacgcag ggcccatcat cgtgcactgc agcgccggca tcggccgcac
     2281 aggcaccatc attgtcatcg acatgctcat ggagaacatc tccaccaagg gcctggactg
     2341 tgacattgac atccagaaga ccatccagat ggtgcgggcg cagcgctcgg gcatggtgca
     2401 gacggaggcg cagtacaagt tcatctacgt ggccatcgcc cagttcattg aaaccactaa
     2461 gaagaagctg gaggtcctgc agtcgcagaa gggccaggag tcggagtacg ggaacatcac
     2521 ctatccccca gccatgaaga atgcccatgc caaggcctcc cgcacctcgt ccaaacacaa
     2581 ggaggatgtg tatgagaacc tgcacactaa gaacaagagg gaggagaaag tgaagaagca
     2641 gcggtcagca gacaaggaga agagcaaggg ttccctcaag aggaagctcg agtacccata
     2701 cgacgtacct gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta
     2761 tgatgtccca gactacgcat gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     2821 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2881 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2941 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3001 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     3061 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3121 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3181 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3241 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3301 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3361 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3421 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3481 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     3541 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3601 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3661 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3721 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3781 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3841 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     3901 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3961 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4021 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4081 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     4141 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4201 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4261 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4321 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4381 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4441 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4501 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4561 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4621 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4681 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4741 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4801 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4861 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     4921 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4981 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5041 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5101 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5161 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5221 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5281 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5341 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5401 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5461 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5521 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5581 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5641 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5701 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5761 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5821 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5881 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5941 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     6001 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6061 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6181 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6241 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6301 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6361 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6421 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6481 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6541 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6601 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6661 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6721 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6781 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6841 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6901 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6961 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7021 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7081 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7141 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7201 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggt
//
