LOCUS V023180 7691 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023180 VERSION V023180 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..3181 /label="QSOX1(NM_002826)" /note="QSOX1(NM_002826)" /gene="QSOX1" CDS 3188..3253 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3291..3515 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3561..3989 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4003..4332 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4399..5193 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5367..5488 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5537..5553) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5561..5577 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5585..5615) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5630..5651 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5939..6524) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6695..7555) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7556..7660) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgaggaggt gcaacagcgg 961 ctccgggccg ccgccgtcgc tgctgctgct gctgctgtgg ctgctcgcgg ttcccggcgc 1021 taacgcggcc ccgcggtcgg cgctctattc gccttccgac ccgctgacgc tgctgcaggc 1081 ggacacggtg cgcggcgcgg tgctgggctc ccgcagcgcc tgggccgtgg agttcttcgc 1141 ctcctggtgc ggccactgca tcgccttcgc cccgacgtgg aaggcgctgg ccgaagacgt 1201 caaagcctgg aggccggccc tgtatctcgc cgccctggac tgtgctgagg agaccaacag 1261 tgcagtctgc agagacttca acatccctgg cttcccgact gtgaggttct tcaaggcctt 1321 taccaagaac ggctcgggag cagtatttcc agtggctggt gctgacgtgc agacactgcg 1381 ggagaggctc attgacgccc tggagtccca tcatgacacg tggcccccag cctgtccccc 1441 actggagcct gccaagctgg aggagattga tggattcttt gcgagaaata acgaagagta 1501 cctggctctg atctttgaaa agggaggctc ctacctgggt agagaggtgg ctctggacct 1561 gtcccagcac aaaggcgtgg cggtgcgcag ggtgctgaac acagaggcca atgtggtgag 1621 aaagtttggt gtcaccgact tcccctcttg ctacctgctg ttccggaatg gctctgtctc 1681 ccgagtcccc gtgctcatgg aatccaggtc cttctatacc gcttacctgc agagactctc 1741 tgggctcacc agggaggctg cccagaccac agttgcacca accactgcta acaagatagc 1801 tcccactgtt tggaaattgg cagatcgctc caagatctac atggctgacc tggaatctgc 1861 actgcactac atcctgcgga tagaagtggg caggttcccg gtcctggaag ggcagcgcct 1921 ggtggccctg aaaaagtttg tggcagtgct ggccaagtat ttccctggcc ggcccttagt 1981 ccagaacttc ctgcactccg tgaatgaatg gctcaagagg cagaagagaa ataaaattcc 2041 ctacagtttc tttaaaactg ccctggacga caggaaagag ggtgccgttc ttgccaagaa 2101 ggtgaactgg attggctgcc aggggagtga gccgcatttc cggggctttc cctgctccct 2161 gtgggtcctc ttccacttct tgactgtgca ggcagctcgg caaaatgtag accactcaca 2221 ggaagcagcc aaggccaagg aggtcctccc agccatccga ggctacgtgc actacttctt 2281 cggctgccga gactgcgcta gccacttcga gcagatggct gctgcctcca tgcaccgggt 2341 ggggagtccc aacgccgctg tcctctggct ctggtctagc cacaacaggg tcaatgctcg 2401 ccttgcaggt gcccccagcg aggaccccca gttccccaag gtgcagtggc caccccgtga 2461 actttgttct gcctgccaca atgaacgcct ggatgtgccc gtgtgggacg tggaagccac 2521 cctcaacttc ctcaaggccc acttctcccc aagcaacatc atcctggact tccctgcagc 2581 tgggtcagct gcccggaggg atgtgcagaa tgtggcagcc gccccagagc tggcgatggg 2641 agccctggag ctggaaagcc ggaattcaac tctggaccct gggaagcctg agatgatgaa 2701 gtcccccaca aacaccaccc cacatgtgcc ggctgaggga cctgaggcaa gtcgaccccc 2761 gaagctgcac cctggcctca gagctgcacc aggccaggag cctcctgagc acatggcaga 2821 gcttcagagg aatgagcagg agcagccgct tgggcagtgg cacttgagca agcgagacac 2881 aggggctgca ttgctggctg agtccagggc tgagaagaac cgcctctggg gccctttgga 2941 ggtcaggcgc gtgggccgca gctccaagca gctggtcgac atccctgagg gccagctgga 3001 ggcccgagct ggacggggcc gaggccagtg gctgcaggtg ctgggagggg gcttctctta 3061 cctggacatc agcctctgtg tggggctcta ttccctgtcc ttcatgggcc tgctggccat 3121 gtacacctac ttccaggcca agataagggc cctgaagggc catgctggcc accctgcagc 3181 cctcgaggac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga 3241 tgacgatgac aagtagtgag ggcccgttta aacccgctga tcagcctcga ctgtgccttc 3301 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 3361 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 3421 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 3481 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 3541 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 3601 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 3661 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 3721 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 3781 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 3841 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 3901 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 3961 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 4021 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 4081 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 4141 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 4201 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 4261 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 4321 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca 4381 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 4441 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 4501 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 4561 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 4621 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 4681 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 4741 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 4801 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 4861 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 4921 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 4981 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 5041 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 5101 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 5161 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 5221 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 5281 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 5341 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 5401 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 5461 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 5521 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 5581 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 5641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 5701 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 5761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 5821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 5881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 5941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 6061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 6121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 6181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 6241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 6301 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 6361 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 6421 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt 6481 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 6541 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 6601 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 6661 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 6721 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6781 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 6841 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 6901 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 6961 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 7021 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 7081 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 7141 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7201 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7261 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7321 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7381 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7441 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 7501 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 7561 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 7621 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 7681 cacctgacgt c //