LOCUS       V035294                10806 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035294
VERSION     V035294
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     regulatory      913..922
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             922..987
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             991..5595
                     /label="Robo2(NM_001358493)"
                     /note="Robo2(NM_001358493)"
                     /gene="Robo2"
     CDS             5602..5655
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5656..6375
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    6406..6630
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6676..7104
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7118..7447
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7514..8308
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8482..8603
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8652..8668)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8676..8692
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8700..8730)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8745..8766
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9054..9639)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9810..10670)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10671..10775)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
      961 atcgactaca aggatgacga tgacaagctt atgttatcat ccgagaaagt ggacaaaatt
     1021 actagactcg aacttctctt cgttttgatg ttattcttct caggaccaac tctaggcaag
     1081 ctgaccagga ccccctggaa gggcaaacct tggaaggggg gatctcgtct tcgtcaagaa
     1141 gactttcccc ccaggatcgt agaacaccct tctgatgtca tcgtctccaa gggagagccc
     1201 accactctga actgtaaagc agagggccga cccaccccca ccattgaatg gtacaaggat
     1261 ggtgagaggg tggagacaga caaggatgat cccaggtccc acagaatgct tctgcccagc
     1321 ggatctttat tctttttgcg aattgttcat gggcgcagaa gtaaaccgga cgaagggagt
     1381 tacgtttgtg ttgcaaggaa ctatcttggt gaagcagtga gtcgaaatgc atctctggaa
     1441 gtggcattat tgcgagatga cttccggcaa aaccccacag atgtggtagt cgcagctgga
     1501 gagcctgcaa tcttggagtg ccagccacca cggggacacc cagaaccaac catctactgg
     1561 aaaaaggaca aagtccgaat tgatgacaag gaagagagaa taagtatccg tggtgggaag
     1621 ctgatgatct ctaatactag gaaaagcgat gctggcatgt acacctgtgt gggaaccaat
     1681 atggtgggag aaagggacag cgaccctgca gagctcactg tctttgaacg acccacattt
     1741 ctcaggaggc caattaacca ggtggtgcta gaggaagaag ctgtagaatt ccgttgtcag
     1801 gtccaaggag atccccagcc aacggtgagg tggaaaaaag atgatgcaga cttgccgaga
     1861 ggaaggtatg atatcaaaga tgactacacg ctgagaatta aaaaggccat gagtactgat
     1921 gaaggtacct atgtgtgtat tgctgagaat cgggtgggaa aagtggaagc ctctgctacc
     1981 ctcactgtcc gagttcgccc tgttgctcct ccacagtttg tggttaggcc aagagatcag
     2041 atcgttgctc aaggccgaac agtgacattc ccctgtgaaa ctaaaggaaa cccacagcca
     2101 gctgtttttt ggcagaaaga aggcagccag aacctacttt tcccgaatca acctcagcag
     2161 cccaacagcc gatgttcagt gtcgcccacg ggggacctca ccatcaccaa catccagcgt
     2221 tcagatgcgg gttactacat ctgccaggcc ctaaccgtgg caggaagcat tttagctaaa
     2281 gcacagttgg aagttactga cgttttgaca gatagacctc cacccataat cttgcaagga
     2341 ccaataaacc aaacacttgc agtagacggt acagcattgt tgaagtgtaa agccactggt
     2401 gagcctctgc ctgtaattag ctggctaaag gagggcttta cttttctggg gagagatcca
     2461 agagccacga tccaagacca aggaacactg cagattaaga atttacggat atctgatact
     2521 ggcacttata cttgtgtggc tacaagttcc agtggagaga cttcctggag tgcagtgctg
     2581 gatgtaacag aatctggagc aacaatcagt aaaaattatg atatgaatga cctcccggga
     2641 ccaccatcca aacctcaggt cactgatgtt tctaagaaca gtgtcacctt atcctggcag
     2701 ccaggtacac ctggcgttct tcctgcaagc gcgtatatca ttgaggcttt cagccaatcg
     2761 gtgagcaata gctggcagac agtggcaaac catgttaaga caactctgta tacagtaagg
     2821 gggctgaggc ccaacacaat ctacttgttt atggtcagag cgatcaaccc acaaggtctc
     2881 agtgatccaa gtcctatgtc ggatcctgta cgcacacaag atatcagccc cccagcacaa
     2941 ggagtggacc acagacaggt gcagaaggaa ttaggtgatg tcgttgttcg tctccataat
     3001 ccagttgtcc tgacacctac aactgttcaa gtcacatgga cggtggaccg acaaccccag
     3061 tttattcagg gctacagagt gatgtaccgt cagacttcgg gactacaagc ctcaactgtg
     3121 tggcagaatc tagacgccaa agtcccgact gagaggagtg ctgtccttgt gaatttgaaa
     3181 aagggggtga cttatgaaat taaagtccgg ccgtatttta acgagttcca aggaatggac
     3241 agtgaatcga aaacagtccg aaccactgag gaagccccaa gtgcccctcc ccagtctgtc
     3301 actgtgctga cagttggaag tcacaacagc acaagcatca gtgtttcctg ggatcctcca
     3361 ccagccgacc accagaatgg aattattcag gaatataaga tctggtgtct gggaaacgaa
     3421 acgcgattcc atatcaataa aacggtggat gcagccattc gctctgtagt aataggtggc
     3481 ttgttccctg gaattcagta ccgggtagaa gtggcagcta gcacaagtgc aggggttgga
     3541 gtaaaaagtg aaccacagcc gataataatt gggggacgta acgaagttgt cattactgaa
     3601 aacaataaca gcatcactga gcaaatcacg gatgtcgtga agcaaccggc atttatagct
     3661 ggcattggtg gtgcctgctg ggtaattctg atgggtttta gcatctggtt gtactggaga
     3721 agaaagaaga gaaagggact cagcaattat gctgtaacat ttcaaagagg agatggagga
     3781 ctaatgagca atgggagccg tccaggtctt ctaaatgctg gcgatcccaa ttacccatgg
     3841 cttgctgatt cttggccagc cacgagtttg ccagtgaaca atagcaatag tggcccaaat
     3901 gaaattggaa attttgggcg tggagatgtg ctgcctccgg tgccaggcca aggggataaa
     3961 acagcgacca tgctctcgga tggagccatt tatagcagca ttgacttcac taccaaaacc
     4021 acttacaaca gttccagcca aataacacag gccaccccat atgccactac acaaatcctg
     4081 cattcaaaca gcatccacga actggcagtt gatcttcctg atccacagtg gaaaagctca
     4141 gttcaacaga agacagacct catgggattt ggttattcgc tacctgatca gaacaagggg
     4201 aacaacgcct tactttacat ccctgactac cgattggctg agggattgtc taatagaatg
     4261 ccacacaacc agtcacagga tttcagcacc accagctctc acaacagctc agaaaggagt
     4321 ggcagtctct caggtgggaa aggtggaaaa aagaagaaaa ctaaaaattc ttcgaaagcg
     4381 cagaaaaaca acggatccac ttgggctaat gtccctctac ctcctcctcc cgtccagccc
     4441 cttcctggta cagagctggg ccactatgct gcggaacaag aaaatggcta tgacagtgac
     4501 agctggtgcc caccattacc ggtgcaaaca tacttgcatc agggtatgga agatgagctg
     4561 gaagaagacg aagatcgggt cccaacacct cctgttcgcg gcgtggcctc ttcaccagct
     4621 atctcttttg gacagcagtc cactgccact cttactccat ccccacggga agagatgcaa
     4681 cccatgctgc aagctcactt ggatgagttg acaagggcct atcagtttga tatagcaaaa
     4741 caaacatggc acattcaaag caatacccca cctccacaac ccccagctcc gccattaggt
     4801 tacgtgtccg gagccttgat ttctgatttg gagacagatg ttccagatga ggatgctgat
     4861 gatgaagagg aaccattaga aattcccagg cccctcagag cactagacca gacacctgga
     4921 tccagtatgg acaatctaga cagctctgtc acaggaaaag cctttagctc ctctcaaagg
     4981 cagcggccca ccagcccatt ttctacggac agtaacacca gtgctgccca gaatcaaagc
     5041 cagaggcctc ggcccacgaa aaaacataag ggaggacgga tggacccaca gccagtgtta
     5101 cctcatcgga gggaagggat gccagatgat cttcccccac caccagatcc cccaccaggt
     5161 cagggtttaa ggcagcagat aggcctgagc caacactctg gtaacgtgga aaactcaaca
     5221 gagagaaaag gaagctctct agagagacaa caagcagcca acttagaaga cacaaagagc
     5281 tcattggatt gtccagctaa aactgttcta gagtggcagc gtcaaaccca ggactggata
     5341 aactctacag aacgccaaga agaaactcgg aaagccccac acaaacaagg ggttggatca
     5401 gaggagtccc tggtgcccta cagcaagccc agcttcccgt ctcctggcgg gcacagctca
     5461 tcaggaacat cttcctctaa agggtccact ggccctcgga aggctgacgt gctgagaggc
     5521 agccaccaga ggaatgccaa tgaccttctc gacattggat atgtgggctc aaatagtcaa
     5581 ggacagttta cagagctcga ggagggcaga ggaagtcttc taacatgcgg tgacgtggag
     5641 gagaatcccg gccctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     5701 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     5761 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     5821 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     5881 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5941 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     6001 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     6061 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     6121 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     6181 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     6241 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     6301 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     6361 gagctgtaca agtagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6421 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6481 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6541 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6601 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6661 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6721 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6781 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6841 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6901 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6961 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7021 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7081 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7141 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7201 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7261 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7321 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7381 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7441 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     7501 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7561 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     7621 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     7681 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     7741 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     7801 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     7861 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7921 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7981 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     8041 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     8101 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     8161 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     8221 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     8281 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     8341 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8401 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8461 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8581 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8641 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8701 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8761 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8821 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8881 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8941 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9001 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9061 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9121 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9181 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9241 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9301 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9361 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9421 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9481 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9541 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
     9601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9661 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9721 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9781 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9841 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9901 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9961 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10021 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10081 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10141 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10201 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10261 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10321 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10381 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10441 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10501 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10561 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10621 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10681 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10741 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10801 gacgtc
//
