LOCUS       V026631                 8282 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026631
VERSION     V026631
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             941..3772
                     /label="Ror2(NM_013846)"
                     /note="Ror2(NM_013846)"
                     /gene="Ror2"
     CDS             3779..3844
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3882..4106
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4152..4580
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4594..4923
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4990..5784
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5958..6079
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6128..6144)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6152..6168
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6176..6206)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6221..6242
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6530..7115)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7286..8146)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8147..8251)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggctcggg gctgggtgcg
      961 gccgagccgt gtgcctctgt gcgcccgggc cgtctggacg gctgcggcgc tcctgctctg
     1021 gacaccctgg acggcaggtg aagtggaaga ttcggaggca atcgacacct tgggacaacc
     1081 tgatggaccg gacagcccac ttcccactct gaaaggctac tttctgaatt ttctggagcc
     1141 agtcaacaat atcaccattg ttcagggcca gacggcaatc ctgcactgca aggtggcggg
     1201 aaacccacct cccaatgtgc ggtggctgaa gaatgatgcc ccggttgtgc aagagccacg
     1261 aagggtcatc atccggaaga cagaatacgg ctcccggctg cggatccaag acctggacac
     1321 aacagacaca ggctactacc agtgtgtggc taccaacggg ctgaagacca tcactgccac
     1381 tggggttcta tatgtgcggc tcggtccgac gcacagcccg aaccacaatt ttcaggatga
     1441 cgatcaggaa gatggcttct gccagccgta ccgagggatc gcttgtgcgc gcttcattgg
     1501 gaaccggact atttatgtgg actccctcca gatgcagggg gagattgaaa accgaatcac
     1561 agctgccttc accatgatcg gcacctccac gcaactgtca gaccagtgtt cacagtttgc
     1621 catcccatcc ttctgccact tcgtcttccc tctgtgcgac gcacgctccc gggcgcccaa
     1681 gcctcgggaa ctgtgccggg atgaatgtga ggtgctggag aacgacctgt gccgccagga
     1741 gtacaccatc gcccgctcca acccgctcat cctcatgcgg ctccagctgc ccaagtgcga
     1801 agcgctgccc atgcccgaga gcccggatgc tgcgaactgc atgcgcatcg ggatcccggc
     1861 ggagaggctg ggtcgctacc accagtgcta caacggctct ggcgccgatt acagggggat
     1921 ggccagtacc accaagtcag gccaccagtg tcagccttgg gctctgcagc acccccacag
     1981 ccatcgccta tccagcacgg aattccctga gctgggagga ggccatgcct actgccggaa
     2041 ccccgggggc cagatggaag gcccgtggtg ctttacgcag aataaaaacg tacgcgtgga
     2101 actgtgtgac gtacccccgt gtagtccccg agatggcagc aagatgggga ttctgtacat
     2161 cctggtcccc agcattgcca tccccctggt catcgcttgc ctgttcttcc tcgtctgcat
     2221 gtgccgcaac aaacagaagg cttcggcctc caccccacag cgccggcagc tgatggcctc
     2281 gcccagccag gacatggaga tgccactcat cagccagcac aaacaggcca aactcaaaga
     2341 gatcagcttg tccacagtga ggttcatgga ggagctcggg gaggaccggt ttggcaaggt
     2401 ctacaaaggc cacctgttcg ggcctgcccc aggagaaccc acccaggccg tggccatcaa
     2461 gacgctgaaa gacaaggctg aggggcccct gcgggaggag ttccggcaag aggcgatgct
     2521 ccgggcccga ctgcagcacc ccaacatcgt ctgcctccta ggcgtcgtga ccaaggacca
     2581 acccttgagc atgatcttca gctactgttc ccatggcgac ctccatgagt tcctggtcat
     2641 gcgctcgccg cactccgatg tgggcagcac cgatgacgac cgcaccgtga agtcagccct
     2701 ggagcccccg gacttcgtgc acgtggtggc gcagatcgct gcggggatgg agttcctgtc
     2761 cagccaccac gtggtccata aggacctggc cacacgcaat gtgctggtgt acgacaagct
     2821 gaacgtgagg atctcagact tgggcctctt ccgtgaggta tactccgcag attactacaa
     2881 actcatgggc aattcactgc tgcccatccg ctggatgtcc cccgaggccg tcatgtatgg
     2941 aaagttctcc atcgactctg acatctggtc ctacggtgtg gtcctctggg aggtctttag
     3001 ctacggcctg cagccctact gtgggtactc caaccaggac gtggtggaga tgatccggag
     3061 ccggcaggtg ctgccctgtc cggatgactg ccccgcctgg gtctatgccc tcatgattga
     3121 atgctggaat gagttcccaa gccggaggcc ccgctttaag gacatccaca gccggctccg
     3181 gtcctggggc aacctatcca actataatag ttccgcgcag acctcaggag ccagcaacac
     3241 cacacagacc agctccctga gcaccagccc tgtaagcaat gtgagcaatg cccgctatat
     3301 ggcccccaag cagaaggccc agcccttccc acagcctcag ttcatcccca tgaagggtca
     3361 gatcagaccc ttggtgcccc cggcacagct gtacatcccg gtgaacggct atcagccggt
     3421 accggcatac ggggcctacc tgcccaactt ctacccagtc cagatcccca tgcagatggc
     3481 cccacagcag gtgccccctc agatggtccc caagccgagc tcacaccaca gtggcagcgg
     3541 ctccaccagc actggctacg tcaccacggc gccctccaat acatctgtgg cggacagggc
     3601 ggccctactc tctgagggca ccgaggatgc acagaacatc gcggaagacg tggcccagag
     3661 ccctgtgcag gaagcagagg aggaggagga ggggtctgtc cctgagactg aactcctggg
     3721 agacaatgac acgctccagg tgaccgaggc ggctcatgtc cagcttgaag ccctcgagga
     3781 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     3841 caagtagtga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3901 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3961 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4021 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4081 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4141 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4201 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4261 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4321 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4381 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4441 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4501 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4561 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4621 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4681 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4741 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4801 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4861 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4921 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     4981 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5041 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5101 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5161 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5221 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5281 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5341 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5401 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5461 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5521 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5581 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5641 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5701 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5761 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5821 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5881 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5941 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6061 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6121 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6181 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6241 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6301 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6361 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6721 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6961 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     7081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8281 tc
//
