LOCUS V040014 7114 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040014 VERSION V040014 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..2604 /label="SCP2(NM_002979)" /note="SCP2(NM_002979)" /gene="SCP2" CDS 2611..2676 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2714..2938 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2984..3412 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3426..3755 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3822..4616 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4790..4911 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4960..4976) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4984..5000 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5008..5038) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5053..5074 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5362..5947) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6118..6978) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6979..7083) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatgtcct cttccccgtg ggagcctgcg accctgcgcc gggtgttcgt ggtgggggtt 1021 ggcatgacca agtttgtgaa gcctggagct gagaattcaa gagactaccc tgacttggca 1081 gaagaagcag gcaagaaggc tttagctgat gcacagatcc cttattcagc agtggaccag 1141 gcatgtgttg gctatgtttt tggtgactct acctgtgggc agagggctat ctatcacagt 1201 ttgggaatga ctggaattcc tataatcaat gtcaacaata actgtgctac tggttctact 1261 gctttgttta tggcccgcca gctgattcag ggtggtgtgg cagaatgtgt cttggctctt 1321 gggtttgaga agatgagtaa gggaagcctt ggaataaaat tttcagatag aaccattccc 1381 actgataagc atgttgacct cctgatcaat aagtatggat tgtctgctca cccagttgct 1441 cctcagatgt ttgggtatgc tggaaaagaa catatggaaa aatatggaac aaaaattgaa 1501 cactttgcaa aaattggatg gaaaaatcat aaacattcag ttaataaccc gtattcccag 1561 ttccaagatg aatacagttt agatgaagtg atggcatcta aagaagtttt tgattttttg 1621 actatcttac aatgttgtcc cacttcagat ggtgctgcag cagcaatttt ggccagtgaa 1681 gcatttgtac agaagtatgg cctgcaatcc aaagctgtgg aaattttggc acaagaaatg 1741 atgactgatt tgccaagctc gtttgaagaa aaaagcatta ttaaaatggt tggctttgat 1801 atgagtaaag aagctgcaag aaaatgctat gagaaatctg gcctgacacc aaatgatatt 1861 gacgtaatag aacttcacga ttgcttttct accaacgaac tccttactta tgaagcactg 1921 ggactctgtc cagaaggaca aggtgcaacg ctggttgata gaggagataa tacatatgga 1981 ggaaagtggg tcataaatcc tagtggtgga ctgatttcaa agggacaccc actaggcgct 2041 acaggtcttg ctcagtgtgc agaactctgc tggcagctga gaggggaagc cggaaagagg 2101 caagttcctg gtgcaaaggt ggctctgcag cataatttag gcattggagg agctgtggtt 2161 gtaacactct acaagatggg ttttccggaa gccgccagtt cttttagaac tcatcaaatt 2221 gaagctgttc caaccagctc tgcaagtgat ggatttaagg caaatcttgt ttttaaggag 2281 attgagaaga aacttgaaga ggaaggggaa cagtttgtga agaaaatcgg tggtattttt 2341 gccttcaagg tgaaagatgg ccctgggggt aaagaggcca cctgggtggt ggatgtgaag 2401 aatggcaaag gatcagtgct tcctaactca gataagaagg ctgactgcac aatcacaatg 2461 gctgactcag acttcctggc tttaatgact ggtaaaatga atcctcagtc ggccttcttt 2521 caaggcaaat tgaaaatcac tggcaacatg ggtctcgcta tgaagttaca aaatcttcag 2581 cttcagccag gcaacgctaa gctcctcgag gactacaaag accatgacgg tgattataaa 2641 gatcatgaca tcgactacaa ggatgacgat gacaagtagt gagggcccgt ttaaacccgc 2701 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 2761 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 2821 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 2881 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 2941 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 3001 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 3061 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 3121 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 3181 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 3241 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 3301 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 3361 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 3421 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3481 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 3541 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 3601 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 3661 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 3721 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 3781 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 3841 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 3901 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 3961 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 4021 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 4081 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 4141 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 4201 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 4261 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 4321 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 4381 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 4441 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 4501 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 4561 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 4621 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 4681 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 4741 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 4801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 4861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 4921 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 4981 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 5041 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 5101 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 5161 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 5221 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5281 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5341 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5401 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5461 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5521 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5581 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5641 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5701 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5761 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 5821 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5881 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 5941 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 6001 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 6061 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 6121 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 6181 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 6241 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6301 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6361 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6421 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 6481 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 6541 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 6601 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 6661 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 6721 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6781 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6841 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6901 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6961 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 7021 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 7081 gcgcacattt ccccgaaaag tgccacctga cgtc //