LOCUS       V046732                 8518 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046732
VERSION     V046732
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             964..4008
                     /label="SKOR2(NM_001278063)"
                     /note="SKOR2(NM_001278063)"
                     /gene="SKOR2"
     CDS             4015..4080
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    4118..4342
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4388..4816
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4830..5159
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5226..6020
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6194..6315
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6364..6380)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6388..6404
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6412..6442)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6457..6478
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6766..7351)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7522..8382)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8383..8487)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatggctt ccagtccgct gccagggccc aacgacatcc tgctggcgtc gccgtcgagc
     1021 gccttccagc ccgacacgct gagccagccg cggccagggc acgccaacct caaacccaac
     1081 caggtgggcc aggtgatcct ctacggcatt cccatcgtgt cgttggtgat cgacgggcaa
     1141 gagcgcctgt gcctggcgca gatctccaac actctgctca agaacttcag ctacaacgag
     1201 atccacaacc gtcgcgtggc actgggcatc acgtgtgtgc agtgcacgcc ggtgcaactg
     1261 gagatcctgc ggcgtgccgg ggccatgccc atctcatcgc gccgctgcgg catgatcacc
     1321 aaacgcgagg ccgagcgtct gtgcaagtcg ttcctgggcg aaaacaggcc gcccaagctg
     1381 ccagacaatt tcgccttcga cgtgtcacac gagtgcgcct ggggctgccg cggcagcttc
     1441 attcccgcgc gctacaacag ctcgcgcgcc aagtgcatca aatgcagcta ctgcaacatg
     1501 tacttctcgc ccaacaagtt cattttccac tcccaccgca cgcccgacgc caagtacact
     1561 cagccagacg cagccaactt caactcgtgg cgccgtcatc tcaagctcac cgacaagagt
     1621 ccccaggacg agctggtctt cgcctgggag gacgtcaagg ccatgttcaa cggcggcagc
     1681 cgcaagcgcg cactgcccca gccgggcgcg caccccgcct gccacccgct cagctctgtg
     1741 aaggcggccg ccgtggccgc cgcggccgcg gtggccggag gcgggggtct gctgggcccc
     1801 cacctgctgg gtgcgccccc gccgccgccg ccgccaccgc cgcccttggc agagctggct
     1861 ggtgccccgc acgcccatca caagcggccg cgcttcgacg acgacgacga ctccttgcag
     1921 gaggccgccg tagtggccgc cgccagcctc tcggccgcag ccgccagcct ctctgtggct
     1981 gctgcttcgg gcggcgcggg gactggtggg ggcggcgctg ggggtggctg tgtggccggc
     2041 gtgggcgtgg gcgcgggcgc gggggcgggt gccggggcag gggccaaagg cccgcgcagc
     2101 tacccagtca tcccggtgcc cagcaagggc tcgttcgggg gcgtcctgca gaagttcccg
     2161 ggctgcggcg ggctcttccc gcacccctac accttccctg ccgcggccgc cgccttcagc
     2221 ttgtgccata agaaagagga tgcgggtgcc gccgctgagg ccctgggggg cgcgggcgca
     2281 ggcggcgcgg gcgcggcgcc caaggccggc ttgtccggcc tcttctggcc cgcgggccgc
     2341 aaggacgcct tctatccgcc cttctgcatg ttctggccgc cgcggacccc tggcgggctc
     2401 ccggtgccca cctacctgca gcccccgcct cagccgccct cggcgctagg ctgcgcgcta
     2461 ggcgaaagcc cggccctgct gcgccaggcc ttcctggacc tggctgagcc aggcggtgct
     2521 gctgggagcg ccgaggccgc gcccccgccg gggcagcccc cgcaggtagt ggccaacggc
     2581 ccgggctccg gcccacctcc tcctgccggg ggcgccggct ctcgcgacgc gctcttcgag
     2641 tcgcccccgg gcggcagcgg cggggactgc agcgcgggct ccacgccgcc cgcggactct
     2701 gtggcagctg ccggggcagg ggccgcggcc gccgggtctg gccccgcggg ctcccgggtt
     2761 ccggcgcccc accatccgca ccttctggag gggcgcaaag cgggcggtgg cagctaccac
     2821 cattccagcg ccttccggcc agtgggcggc aaggacgacg cggagagcct ggccaagctg
     2881 cacggggcgt cggcgggcgc gccccactcg gcccagacgc atccccacca ccatcaccac
     2941 cctcaccacc accaccacca ccaccacccc ccgcagccgc cgtcgccgct tctgctgctg
     3001 cccccgcagc ccgacgagcc gggttccgag cgccaccacc cggccccgcc gccgccgccg
     3061 ccgccgcccc cgccgccccc tctggcccag cacccgcacc accgaggcct tctgtctccc
     3121 gggggaacca gctgctgcta ccccagcgag gacagctccg aggacgagga cgacgaggaa
     3181 gaagagcagg aggtggacgt ggagggccac aagccccccg agggcgagga agaggaggaa
     3241 ggtcgagacc ctgacgacga cgaggaagag gacgaggaga cggaggtcct actcggcgac
     3301 cccttagtcg ggggcggccg gttcctccag ggccgagggc cgtcggagaa ggggagcagc
     3361 cgggaccgcg cgccggccgt cgcgggcgcg ttcccgctcg gcctgaactc ctccaggctg
     3421 ctgcaggaag acgggaaact cggggacccc ggctcggacc tgcccccgcc cccgccgccg
     3481 cccctggccc cccagaaggc gagtggcggc ggcagcagca gcccgggcag cccagttcac
     3541 catccatcac tggaggagca gccctcctac aaagatagtc agaaaactaa ggaaaataac
     3601 caagtaattg tatctacaaa ggatgacaac agcttttcag ataagaacaa ggagcatagc
     3661 tttttcatca cagactctga tgcttctgga ggagattttt ggagagaaag atcaggtgaa
     3721 catacacaag aaaccaactc acctcattca ctgaaaaagg atgtagaaaa tatggggaaa
     3781 gaagaacttc agaaggtttt atttgaacaa atagatttac ggagacgact ggaacaagaa
     3841 ttccaggtgt taaaaggaaa cacatctttc ccagtattca ataattttca ggatcagatg
     3901 aaaagggagc tagcctaccg agaagaaatg gtgcaacagt tacaaattat cccctatgca
     3961 gcaagtttga tcaggaaaga aaagcttggc gcccatctca gcaaaagcct cgaggactac
     4021 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     4081 tagtgagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4141 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4201 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4261 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4321 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4381 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4441 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4501 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4561 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4621 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4681 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4741 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4801 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4861 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4921 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4981 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5041 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     5101 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5161 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     5221 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5281 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5341 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5401 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5461 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5521 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5581 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5641 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5701 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5761 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5821 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5881 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5941 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6001 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     6061 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6121 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6181 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6241 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6301 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6361 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6421 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6481 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6541 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6601 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6661 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6781 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6841 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6901 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6961 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7021 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7081 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7141 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7201 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7261 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca
     7321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7441 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7501 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7561 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7621 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7681 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7741 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7801 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7861 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7921 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7981 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8041 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8101 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8161 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8221 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8281 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8341 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
