LOCUS       V025981                 7973 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025981
VERSION     V025981
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             941..3463
                     /label="SLITRK6(NM_032229)"
                     /note="SLITRK6(NM_032229)"
                     /gene="SLITRK6"
     CDS             3470..3535
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3573..3797
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3843..4271
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4285..4614
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4681..5475
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5649..5770
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5819..5835)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5843..5859
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5867..5897)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5912..5933
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6221..6806)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6977..7837)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7838..7942)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgaagctgt ggattcatct
      961 cttttattca tctctccttg cctgtatatc tttacactcc caaactccag tgctctcatc
     1021 cagaggctct tgtgattctc tttgcaattg tgaggaaaaa gatggcacaa tgctaataaa
     1081 ttgtgaagca aaaggtatca agatggtatc tgaaataagt gtgccaccat cacgaccttt
     1141 ccaactaagc ttattaaata acggcttgac gatgcttcac acaaatgact tttctgggct
     1201 taccaatgct atttcaatac accttggatt taacaatatt gcagatattg agataggtgc
     1261 atttaatggc cttggcctcc tgaaacaact tcatatcaat cacaattctt tagaaattct
     1321 taaagaggat actttccatg gactggaaaa cctggaattc ctgcaagcag ataacaattt
     1381 tatcacagtg attgaaccaa gtgcctttag caagctcaac agactcaaag tgttaatttt
     1441 aaatgacaat gctattgaga gtcttcctcc aaacatcttc cgatttgttc ctttaaccca
     1501 tctagatctt cgtggaaatc aattacaaac attgccttat gttggttttc tcgaacacat
     1561 tggccgaata ttggatcttc agttggagga caacaaatgg gcctgcaatt gtgacttatt
     1621 gcagttaaaa acttggttgg agaacatgcc tccacagtct ataattggtg atgttgtctg
     1681 caacagccct ccatttttta aaggaagtat actcagtaga ctaaagaagg aatctatttg
     1741 ccctactcca ccagtgtatg aagaacatga ggatccttca ggatcattac atctggcagc
     1801 aacatcttca ataaatgata gtcgcatgtc aactaagacc acgtccattc taaaactacc
     1861 caccaaagca ccaggtttga taccttatat tacaaagcca tccactcaac ttccaggacc
     1921 ttactgccct attccttgta actgcaaagt cctatcccca tcaggacttc taatacattg
     1981 tcaggagcgc aacattgaaa gcttatcaga tctgagacct cctccgcaaa atcctagaaa
     2041 gctcattcta gcgggaaata ttattcacag tttaatgaag tctgatctag tggaatattt
     2101 cactttggaa atgcttcact tgggaaacaa tcgtattgaa gttcttgaag aaggatcgtt
     2161 tatgaaccta acgagattac aaaaactcta tctaaatggt aaccacctga ccaaattaag
     2221 taaaggcatg ttccttggtc tccataatct tgaatactta tatcttgaat acaatgccat
     2281 taaggaaata ctgccaggaa cctttaatcc aatgcctaaa cttaaagtcc tgtatttaaa
     2341 taacaacctc ctccaagttt taccaccaca tattttttca ggggttcctc taactaaggt
     2401 aaatcttaaa acaaaccagt ttacccatct acctgtaagt aatattttgg atgatcttga
     2461 tttgctaacc cagattgacc ttgaggataa cccctgggac tgctcctgtg acctggttgg
     2521 actgcagcaa tggatacaaa agttaagcaa gaacacagtg acagatgaca tcctctgcac
     2581 ttcccccggg catctcgaca aaaaggaatt gaaagcccta aatagtgaaa ttctctgtcc
     2641 aggtttagta aataacccat ccatgccaac acagactagt taccttatgg tcaccactcc
     2701 tgcaacaaca acaaatacgg ctgatactat tttacgatct cttacggacg ctgtgccact
     2761 gtctgttcta atattgggac ttctgattat gttcatcact attgttttct gtgctgcagg
     2821 gatagtggtt cttgttcttc accgcaggag aagatacaaa aagaaacaag tagatgagca
     2881 aatgagagac aacagtcctg tgcatcttca gtacagcatg tatggccata aaaccactca
     2941 tcacactact gaaagaccct ctgcctcact ctatgaacag cacatggtga gccccatggt
     3001 tcatgtctat agaagtccat cctttggtcc aaagcatctg gaagaggaag aagagaggaa
     3061 tgagaaagaa ggaagtgatg caaaacatct ccaaagaagt cttttggaac aggaaaatca
     3121 ttcaccactc acagggtcaa atatgaaata caaaaccacg aaccaatcaa cagaattttt
     3181 atccttccaa gatgccagct cattgtacag aaacatttta gaaaaagaaa gggaacttca
     3241 gcaactggga atcacagaat acctaaggaa aaacattgct cagctccagc ctgatatgga
     3301 ggcacattat cctggagccc acgaagagct gaagttaatg gaaacattaa tgtactcacg
     3361 tccaaggaag gtattagtgg aacagacaaa aaatgagtat tttgaactta aagctaattt
     3421 acatgctgaa cctgactatt tagaagtcct ggagcagcaa acactcgagg actacaaaga
     3481 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtagtg
     3541 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3601 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3661 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3721 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3781 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3841 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3901 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3961 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4021 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4081 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4141 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4201 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4261 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4321 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4381 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4441 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4501 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4561 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4621 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     4681 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4741 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4801 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4861 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4921 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4981 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5041 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5101 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5161 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5221 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5281 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5341 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5401 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5461 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5521 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5581 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5641 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5701 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5761 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     5821 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5881 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5941 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6001 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6061 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6121 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6181 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6241 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6301 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6361 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6421 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6481 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6541 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6601 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6661 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6721 gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga
     6781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7261 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7501 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7561 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7621 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7681 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7741 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7801 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7861 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7921 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
