LOCUS pcDNA3.1-SPP1(human) 6363 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044819 VERSION V044819 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 955..1896 /label="SPP1(NM_001040058)" /note="SPP1(NM_001040058)" /gene="SPP1" CDS 1912..1929 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" polyA_signal 1958..2182 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2228..2656 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2670..2999 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3066..3860 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4036..4157 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4206..4222) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4230..4246 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4254..4284) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4299..4320 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4608..5196) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5367..6227) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6228..6332) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatgaga 961 attgcagtga tttgcttttg cctcctaggc atcacctgtg ccataccagt taaacaggct 1021 gattctggaa gttctgagga aaagcagctt tacaacaaat acccagatgc tgtggccaca 1081 tggctaaacc ctgacccatc tcagaagcag aatctcctag ccccacagaa tgctgtgtcc 1141 tctgaagaaa ccaatgactt taaacaagag acccttccaa gtaagtccaa cgaaagccat 1201 gaccacatgg atgatatgga tgatgaagat gatgatgacc atgtggacag ccaggactcc 1261 attgactcga acgactctga tgatgtagat gacactgatg attctcacca gtctgatgag 1321 tctcaccatt ctgatgaatc tgatgaactg gtcactgatt ttcccacgga cctgccagca 1381 accgaagttt tcactccagt tgtccccaca gtagacacat atgatggccg aggtgatagt 1441 gtggtttatg gactgaggtc aaaatctaag aagtttcgca gacctgacat ccagtaccct 1501 gatgctacag acgaggacat cacctcacac atggaaagcg aggagttgaa tggtgcatac 1561 aaggccatcc ccgttgccca ggacctgaac gcgccttctg attgggacag ccgtgggaag 1621 gacagttatg aaacgagtca gctggatgac cagagtgctg aaacccacag ccacaagcag 1681 tccagattat ataagcggaa agccaatgat gagagcaatg agcattccga tgtgattgat 1741 agtcaggaac tttccaaagt cagccgtgaa ttccacagcc atgaatttca cagccatgaa 1801 gatatgctgg ttgtagaccc caaaagtaag gaagaagata aacacctgaa atttcgtatt 1861 tctcatgaat tagatagtgc atcttctgag gtcaattcta gagggccctt ccatcatcac 1921 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 1981 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 2041 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 2101 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 2161 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 2221 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 2281 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 2341 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 2401 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 2461 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 2521 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 2581 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 2641 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 2701 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 2761 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 2821 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 2881 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 2941 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 3001 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt 3061 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 3121 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 3181 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 3241 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 3301 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 3361 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 3421 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 3481 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 3541 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc 3601 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 3661 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 3721 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 3781 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 3841 ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg 3901 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 3961 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4021 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 4081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 4141 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 4201 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 4261 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 4321 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 4381 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 4441 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4501 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4561 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 4621 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 4681 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 4741 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 4801 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 4861 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 4921 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 4981 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 5041 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 5101 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 5161 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5221 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5281 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5341 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5401 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5461 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5521 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 5581 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 5641 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 5701 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 5761 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 5821 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 5881 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 5941 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6001 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6061 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6121 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6181 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6241 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6301 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6361 gtc //