LOCUS V026527 6186 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026527 VERSION V026527 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..1645 /label="TFAM(NM_003201)" /note="TFAM(NM_003201)" /gene="TFAM" CDS 1646..1675 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" polyA_signal 1753..1977 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2023..2451 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2465..2794 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2861..3655 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3829..3950 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3999..4015) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4023..4039 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4047..4077) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4092..4113 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4401..4986) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5157..6017) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6018..6122) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gcgtttctcc gaagcatgtg gggcgtgctg agtgccctgg gaaggtctgg 961 agcagagctg tgcaccggct gtggaagtcg actgcgctcc cccttcagtt ttgtgtattt 1021 accgaggtgg ttttcatctg tcttggcaag ttgtccaaag aaacctgtaa gttcttacct 1081 tcgattttct aaagaacaac tacccatatt taaagctcag aacccagatg caaaaactac 1141 agaactaatt agaagaattg cccagcgttg gagggaactt cctgattcaa agaaaaaaat 1201 atatcaagat gcttataggg cggagtggca ggtatataaa gaagagataa gcagatttaa 1261 agaacagcta actccaagtc agattatgtc tttggaaaaa gaaatcatgg acaaacattt 1321 aaaaaggaaa gctatgacaa aaaaaaaaga gttaacactg cttggaaaac caaaaagacc 1381 tcgttcagct tataacgttt atgtagctga aagattccaa gaagctaagg gtgattcacc 1441 gcaggaaaag ctgaagactg taaaggaaaa ctggaaaaat ctgtctgact ctgaaaagga 1501 attatatatt cagcatgcta aagaggacga aactcgttat cataatgaaa tgaagtcttg 1561 ggaagaacaa atgattgaag ttggacgaaa ggatcttcta cgtcgcacaa taaagaaaca 1621 acgaaaatat ggtgctgagg agtgtgagca gaagctgatc tcagaggagg acctgtgaat 1681 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct 1741 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 1801 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 1861 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 1921 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 1981 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 2041 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 2101 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 2161 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 2221 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 2281 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 2341 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 2401 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 2461 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 2521 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 2581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 2641 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2701 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 2761 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 2821 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 2881 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 2941 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 3001 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 3061 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 3121 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 3181 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 3241 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 3301 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 3361 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 3421 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 3481 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 3541 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 3601 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 3661 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 3721 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 3781 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 3841 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 3901 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 3961 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 4021 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 4081 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 4141 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 4201 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 4261 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 4321 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 4381 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 4441 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 4501 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 4561 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 4621 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4681 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4741 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4801 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 4861 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4921 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 4981 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 5041 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 5101 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 5161 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 5221 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 5281 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 5341 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 5401 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 5461 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 5521 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 5581 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 5641 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 5701 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 5761 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 5821 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 5881 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 5941 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 6001 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 6061 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 6121 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc 6181 tatggt //