LOCUS V027350 7852 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027350 VERSION V027350 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..3288 /label="TLR1(NM_003263)" /note="TLR1(NM_003263)" /gene="TLR1" CDS 3295..3384 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 3419..3643 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3689..4117 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4131..4460 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4527..5321 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5495..5616 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5665..5681) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5689..5705 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5713..5743) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5758..5779 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6067..6652) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6823..7683) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7684..7788) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgactagca tcttccattt tgccattatc 961 ttcatgttaa tacttcagat cagaatacaa ttatctgaag aaagtgaatt tttagttgat 1021 aggtcaaaaa acggtctcat ccacgttcct aaagacctat cccagaaaac aacaatctta 1081 aatatatcgc aaaattatat atctgagctt tggacttctg acatcttatc actgtcaaaa 1141 ctgaggattt tgataatttc tcataataga atccagtatc ttgatatcag tgttttcaaa 1201 ttcaaccagg aattggaata cttggatttg tcccacaaca agttggtgaa gatttcttgc 1261 caccctactg tgaacctcaa gcacttggac ctgtcattta atgcatttga tgccctgcct 1321 atatgcaaag agtttggcaa tatgtctcaa ctaaaatttc tggggttgag caccacacac 1381 ttagaaaaat ctagtgtgct gccaattgct catttgaata tcagcaaggt cttgctggtc 1441 ttaggagaga cttatgggga aaaagaagac cctgagggcc ttcaagactt taacactgag 1501 agtctgcaca ttgtgttccc cacaaacaaa gaattccatt ttattttgga tgtgtcagtc 1561 aagactgtag caaatctgga actatctaat atcaaatgtg tgctagaaga taacaaatgt 1621 tcttacttcc taagtattct ggcgaaactt caaacaaatc caaagttatc aaatcttacc 1681 ttaaacaaca ttgaaacaac ttggaattct ttcattagga tcctccagct ggtttggcat 1741 acaactgtat ggtatttctc aatttcaaac gtgaagctac agggtcagct ggacttcaga 1801 gattttgatt attctggcac ttccttgaag gccttgtcta tacaccaagt tgtcagcgat 1861 gtgttcggtt ttccgcaaag ttatatctat gaaatctttt cgaatatgaa catcaaaaat 1921 ttcacagtgt ctggtacacg catggtccac atgctttgcc catccaaaat tagcccgttc 1981 ctgcatttgg atttttccaa taatctctta acagacacgg tttttgaaaa ttgtgggcac 2041 cttactgagt tggagacact tattttacaa atgaatcaat taaaagaact ttcaaaaata 2101 gctgaaatga ctacacagat gaagtctctg caacaattgg atattagcca gaattctgta 2161 agctatgatg aaaagaaagg agactgttct tggactaaaa gtttattaag tttaaatatg 2221 tcttcaaata tacttactga cactattttc agatgtttac ctcccaggat caaggtactt 2281 gatcttcaca gcaataaaat aaagagcatt cctaaacaag tcgtaaaact ggaagctttg 2341 caagaactca atgttgcttt caattcttta actgaccttc ctggatgtgg cagctttagc 2401 agcctttctg tattgatcat tgatcacaat tcagtttccc acccatcggc tgatttcttc 2461 cagagctgcc agaagatgag gtcaataaaa gcaggggaca atccattcca atgtacctgt 2521 gagctaggag aatttgtcaa aaatatagac caagtatcaa gtgaagtgtt agagggctgg 2581 cctgattctt ataagtgtga ctacccggaa agttatagag gaaccctact aaaggacttt 2641 cacatgtctg aattatcctg caacataact ctgctgatcg tcaccatcgt tgccaccatg 2701 ctggtgttgg ctgtgactgt gacctccctc tgcagctact tggatctgcc ctggtatctc 2761 aggatggtgt gccagtggac ccagacccgg cgcagggcca ggaacatacc cttagaagaa 2821 ctccaaagaa atctccagtt tcatgcattt atttcatata gtgggcacga ttctttctgg 2881 gtgaagaatg aattattgcc aaacctagag aaagaaggta tgcagatttg ccttcatgag 2941 agaaactttg ttcctggcaa gagcattgtg gaaaatatca tcacctgcat tgagaagagt 3001 tacaagtcca tctttgtttt gtctcccaac tttgtccaga gtgaatggtg ccattatgaa 3061 ctctactttg cccatcacaa tctctttcat gaaggatcta atagcttaat cctgatcttg 3121 ctggaaccca ttccgcagta ctccattcct agcagttatc acaagctcaa aagtctcatg 3181 gccaggagga cttatttgga atggcccaag gaaaagagca aacgtggcct tttttgggct 3241 aacttaaggg cagccattaa tattaagctg acagagcaag caaagaaact cgaggaacaa 3301 aaactaatat cagaggaaga tttggaacag aaactaatca gtgaagaaga tttagagcaa 3361 aagttaattt ccgaggagga cttatgaggg cccgtttaaa cccgctgatc agcctcgact 3421 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 3481 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 3541 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 3601 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 3661 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 3721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 3781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 3841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 3901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 3961 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 4021 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 4081 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 4141 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 4201 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 4261 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 4321 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 4381 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 4441 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 4501 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 4561 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 4621 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 4681 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 4741 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 4801 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 4861 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 4921 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 4981 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 5041 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 5101 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 5161 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 5221 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 5281 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 5341 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 5401 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 5461 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 5521 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 5581 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc 5641 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 5701 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 5761 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 5821 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 5881 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 5941 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 6001 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 6061 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 6121 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6181 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6241 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6301 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6361 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6421 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6481 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 6541 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 6601 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6661 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6721 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6781 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 6841 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6901 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6961 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7021 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 7081 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 7141 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7201 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7261 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7321 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7381 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 7441 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7501 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7561 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7621 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 7681 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7741 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7801 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gt //