LOCUS       V039646                13274 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039646
VERSION     V039646
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             923..1639
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1676..8764
                     /label="TPR(NM_003292)"
                     /note="TPR(NM_003292)"
                     /gene="TPR"
     CDS             8771..8836
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    8874..9098
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      9144..9572
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9586..9915
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9982..10776
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10950..11071
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11120..11136)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11144..11160
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11168..11198)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11213..11234
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11522..12107)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12278..13138)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13139..13243)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      961 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1021 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1081 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1141 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1201 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1261 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1321 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1381 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1441 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1501 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1561 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1621 catggacgag ctgtacaagg gcggtggagg cagtggtgga ggcggtagcg aattcatggc
     1681 ggcggtgttg cagcaagtcc tggagcgcac ggagctgaac aagctgccca agtctgtcca
     1741 gaacaaactt gaaaagttcc ttgctgatca gcaatccgag atcgatggcc tgaaggggcg
     1801 gcatgagaaa tttaaggtgg agagcgaaca acagtatttt gaaatagaaa agaggttgtc
     1861 ccacagtcag gagagacttg tgaatgaaac ccgagagtgt caaagcttgc ggcttgagct
     1921 agagaaactc aacaatcaac tgaaggcact aactgagaaa aacaaagaac ttgaaattgc
     1981 tcaggatcgc aatattgcca ttcagagcca atttacaaga acaaaggaag aattagaagc
     2041 tgagaaaaga gacttaatta gaaccaatga gagactatct caagaacttg aatacttaac
     2101 agaggatgtt aaacgtctga atgaaaaact taaagaaagc aatacaacaa agggtgaact
     2161 tcagttaaaa ttggatgaac ttcaagcttc tgatgtttct gttaagtatc gagaaaaacg
     2221 cttggagcaa gaaaaggaat tgctacatag tcagaataca tggctgaata cagagttgaa
     2281 aaccaaaact gatgaacttc tggctcttgg aagagaaaaa gggaatgaga ttctagagct
     2341 taaatgtaat cttgaaaata aaaaagaaga ggtttctaga ctggaagaac aaatgaatgg
     2401 cttaaaaaca tcaaatgaac atcttcaaaa gcatgtggag gatctgttga ccaaattaaa
     2461 agaggccaag gaacaacagg ccagtatgga agagaaattc cacaatgaat taaatgccca
     2521 cataaaactt tctaatttgt acaagagtgc cgctgatgac tcagaagcaa agagcaatga
     2581 actaacccgg gcagtagagg aactacacaa acttttgaaa gaagctggtg aagccaacaa
     2641 agcaatacaa gatcatcttc tagaggtgga gcaatccaaa gatcaaatgg aaaaagaaat
     2701 gcttgagaaa atagggagat tggagaagga attagagaat gcaaatgacc ttctttctgc
     2761 cacaaaacgt aaaggagcca tattgtctga agaagagctt gccgccatgt ctcctactgc
     2821 agcagctgta gctaagatag tgaaacctgg gatgaaacta actgagctct ataatgctta
     2881 tgtggaaact caggatcagt tgcttttgga gaaactagag aacaaaagaa ttaataagta
     2941 cctagatgaa atagtgaaag aagtggaagc caaagcacca attttgaaac gccagcgtga
     3001 ggaatatgaa cgtgcacaga aagctgtagc aagtttatct gttaagcttg aacaagctat
     3061 gaaggagatt cagcgattgc aggaggacac tgataaagcc aacaagcaat catctgtact
     3121 tgagagagat aatcgaagaa tggaaataca agtaaaagat ctttcacaac agattagagt
     3181 gcttttgatg gaacttgaag aagcaagggg taaccacgta attcgtgatg aggaagtaag
     3241 ctctgctgat ataagtagtt catctgaggt aatatcacag catctagtat cttacagaaa
     3301 tattgaagag cttcaacaac aaaatcaacg tctcttagtg gcccttagag agcttgggga
     3361 aaccagagaa agagaagaac aagaaacaac ttcatccaaa atcactgagc ttcagctcaa
     3421 acttgagagt gcccttactg aactagaaca actccgcaaa tcacgacagc atcaaatgca
     3481 gcttgttgat tccatagttc gtcagcgtga tatgtaccgt attttattgt cacaaacaac
     3541 aggagttgcc attccattac atgcttcaag cttagatgat gtttctcttg catcaactcc
     3601 aaaacgtcca agtacatcac agactgtttc cactcctgct ccagtacctg ttattgaatc
     3661 aacagaggct atagaggcta aggctgccct taaacagttg caggaaattt ttgagaacta
     3721 caaaaaagaa aaagcagaaa atgaaaaaat acaaaatgag cagcttgaga aacttcaaga
     3781 acaagttaca gatttgcgat cacaaaatac caaaatttct acccagctag attttgcttc
     3841 taaacgttat gaaatgctgc aagataatgt tgaaggatat cgtcgagaaa taacatcact
     3901 tcatgagaga aatcagaaac tcactgccac aactcaaaag caagaacaga ttatcaatac
     3961 gatgactcaa gatttgagag gagcaaatga gaagctagct gtcgcagaag taagagcaga
     4021 aaatttgaag aaggaaaagg aaatgcttaa attgtctgaa gttcgtcttt ctcagcaaag
     4081 agagtctttg ttagctgaac aaagggggca aaacttactg ctaactaatc tgcaaacaat
     4141 tcagggaata ctggagcgat ctgaaacaga aaccaaacaa aggcttagta gccagataga
     4201 aaaactggaa catgagatct ctcatctaaa gaagaagttg gaaaatgagg tggaacaaag
     4261 gcatacactt actagaaatc tagatgttca acttttagat acaaagagac aactggatac
     4321 agagacaaat cttcatctta acacaaaaga actattaaaa aatgctcaaa aagaaattgc
     4381 cacattgaaa cagcacctca gtaatatgga agtccaagtt gcttctcagt cttcacagag
     4441 aactggtaaa ggtcagccta gcaacaaaga agatgtggat gatcttgtga gtcagctaag
     4501 acagacagaa gagcaggtga atgacttaaa ggagagactc aaaacaagta cgagcaatgt
     4561 ggaacaatat caagcaatgg ttactagttt agaagaatcc ctgaacaagg aaaaacaggt
     4621 gacagaagaa gtgcgtaaga atattgaagt tcgtttaaaa gagtcagctg aatttcagac
     4681 acagttggaa aagaagttga tggaagtaga gaaggaaaaa caagaacttc aggatgataa
     4741 aagaagagcc atagagagca tggaacaaca gttatctgaa ttgaagaaaa cactttctag
     4801 tgttcagaat gaagtacaag aagctcttca gagagcaagc acagctttaa gtaatgagca
     4861 gcaagccaga cgtgactgtc aggaacaagc taaaatagct gtggaagctc agaataagta
     4921 tgagagagaa ttgatgctgc atgctgctga tgttgaagct ctacaagctg cgaaggagca
     4981 ggtttcaaaa atggcatcag tccgtcagca tttggaagaa acaacacaga aagcagaatc
     5041 acagttgttg gagtgtaaag catcttggga ggaaagagag agaatgttaa aggatgaagt
     5101 ttccaaatgt gtatgtcgct gtgaagatct ggagaaacaa aacagattac ttcatgatca
     5161 gatcgaaaaa ttaagtgaca aggtcgttgc ctctgtgaag gaaggtgtac aaggtccact
     5221 gaatgtatct ctcagtgaag aaggaaaatc tcaagaacaa attttggaaa ttctcagatt
     5281 tatacgacga gaaaaagaaa ttgctgaaac taggtttgag gtggctcagg ttgagagtct
     5341 gcgttatcga caaagggttg aacttttaga aagagagctg caggaactgc aagatagtct
     5401 aaatgctgaa agggagaaag tccaggtaac tgcaaaaaca atggctcagc atgaagaact
     5461 gatgaagaaa actgaaacaa tgaatgtagt tatggagacc aataaaatgc taagagaaga
     5521 gaaggagaga ctagaacagg atctacagca aatgcaagca aaggtgagga aactggagtt
     5581 agatatttta cccttacaag aagcaaatgc tgagctgagt gagaaaagcg gtatgttgca
     5641 ggcagagaag aagctcttag aagaggatgt caaacgttgg aaagcacgta accagcatct
     5701 agtaagtcaa cagaaagatc cagatacaga agaatatcgg aagctccttt ctgaaaagga
     5761 agttcatact aagcgtattc aacaattgac agaagaaatt ggtagactta aagctgaaat
     5821 tgcaagatca aatgcatctt tgactaacaa ccagaactta attcagagtc tgaaggaaga
     5881 tctaaataaa gtaagaactg aaaaggaaac catccagaag gacttagatg ccaaaataat
     5941 tgatatccaa gaaaaagtca aaactattac tcaagttaag aaaattggac gtaggtacaa
     6001 gactcaatat gaagaactta aagcacaaca ggataaggtt atggagacat cggctcagtc
     6061 ctctggagac catcaggagc agcatgtttc agtccaggaa atgcaggaac tcaaagaaac
     6121 gctcaaccaa gctgaaacaa aatcaaaatc acttgaaagt caagtagaga atctgcagaa
     6181 gacattatct gaaaaagaga cagaagcaag aaatctccag gaacagactg tgcaacttca
     6241 gtctgaactt tcacgacttc gtcaggatct tcaagataga accacacagg aggagcagct
     6301 ccgacaacag ataactgaaa aggaagaaaa aaccagaaag gctattgtag cagcaaagtc
     6361 aaaaattgca cacttagctg gtgtaaaaga tcagctaact aaagaaaatg aggagcttaa
     6421 acaaaggaat ggagccttag atcagcagaa agatgaattg gatgttcgca ttactgcgct
     6481 aaagtcccaa tatgaaggtc gaattagtcg cttggaaaga gaactcaggg agcatcaaga
     6541 gagacacctt gagcagagag atgagcctca agaaccttct aataaggtcc ctgaacagca
     6601 gagacagatc acattgaaaa caactccagc ttctggtgaa agaggaattg ccagcacatc
     6661 agacccacca acagccaata tcaagccaac tcctgttgtg tctactccaa gtaaagtgac
     6721 agctgcagct atggctggaa ataagtcaac acccagggct agtatccgcc caatggttac
     6781 acctgcaact gttacaaatc ccactactac cccaacagct acagtgatgc ccactacaca
     6841 agtggaatca caggaagcta tgcagtcaga agggcctgtg gaacatgttc cagtttttgg
     6901 aagcacaagt ggatccgttc gttctactag tcctaatgtc cagccttcta tctctcaacc
     6961 tattttaact gttcagcaac aaacacaggc tacagctttt gtgcaaccca ctcaacagag
     7021 tcatcctcag attgagcctg ccaatcaaga gttatcttca aacatagtag aggttgttca
     7081 gagttcacca gttgagcggc cttctacttc cacagcagta tttggcacag tttcggctac
     7141 ccccagttct tctttgccaa agcgtacacg tgaagaggaa gaggatagca ccatagaagc
     7201 atcagaccaa gtctctgatg atacagtgga aatgcctctt ccaaagaagt tgaaaagtgt
     7261 cacacctgta ggaactgagg aagaagttat ggcagaagaa agtactgatg gagaggtaga
     7321 gactcaggta tacaaccagg attctcaaga ttccattgga gaaggagtta cccagggaga
     7381 ttatacacct atggaagaca gtgaagaaac ctctcagtct ctacaaatag atcttgggcc
     7441 acttcaatca gatcagcaga cgacaacttc atcccaggat ggtcaaggca aaggagatga
     7501 tgtcattgta attgacagtg atgatgaaga agaggatgat gatgaaaatg atggagaaca
     7561 tgaggattat gaagaggatg aggaagatga tgatgatgat gaagatgaca cagggatggg
     7621 agatgagggt gaagatagta atgaaggaac tggtagtgcc gatggcaatg atggttatga
     7681 agctgatgat gctgagggtg gtgatgggac tgatccaggt acagaaacag aagaaagtat
     7741 gggtggaggt gaaggtaatc acagagctgc tgattctcaa aacagtggtg aaggaaatac
     7801 aggtgctgca gaatcttctt tttctcagga ggtttctaga gaacaacagc catcatcagc
     7861 atctgaaaga caggcccctc gagcacctca gtcaccgaga cgcccaccac atccacttcc
     7921 cccaagactg accattcatg ccccacctca ggagttggga ccaccagttc agagaattca
     7981 gatgacccga aggcagtctg taggacgtgg ccttcagttg actccaggaa taggtggcat
     8041 gcaacagcat ttttttgatg atgaagacag aacagttcca agtactccaa ctcttgtggt
     8101 gccacatcgt actgatggat ttgctgaagc aattcattcg ccgcaggttg ctggtgtccc
     8161 tagattccgg tttgggccac ctgaagatat gccacaaaca agttctagtc actctgatct
     8221 tggccagctt gcttctcaag gaggtttagg aatgtatgaa acacccctgt tcctagctca
     8281 tgaagaagag tcaggtggcc gaagtgttcc cactactcca ctacaagtag cagccccagt
     8341 gactgtattt actgagagca ccacctctga tgcttcggaa catgcctctc aatctgttcc
     8401 aatggtgact acatccactg gcactttatc tacaacaaat gaaacagcaa caggtgatga
     8461 tggagatgaa gtatttgtgg aggcagaatc tgaaggtatt agttcagaag caggcctaga
     8521 aattgatagc cagcaggaag aagagccggt tcaagcatct gatgagtcag atctcccctc
     8581 caccagccag gatcctcctt ctagctcatc tgtagatact agtagtagtc aaccaaagcc
     8641 tttcagacga gtaagacttc agacaacatt gagacaaggt gtccgtggtc gtcagtttaa
     8701 cagacagaga ggtgtgagcc atgcaatggg agggagagga ggaataaaca gaggaaatat
     8761 taatctcgag gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     8821 ggatgacgat gacaagtagt gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     8881 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     8941 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     9001 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     9061 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     9121 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     9181 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     9241 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9301 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     9361 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9421 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9481 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9541 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     9601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9661 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9721 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9781 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9841 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9901 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     9961 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
    10021 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
    10081 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
    10141 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
    10201 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
    10261 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
    10321 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
    10381 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
    10441 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
    10501 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
    10561 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
    10621 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
    10681 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    10741 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
    10801 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
    10861 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
    10921 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
    10981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    11041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
    11101 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    11161 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    11221 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    11281 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    11341 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    11401 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    11461 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    11521 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    11581 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    11641 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    11701 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    11761 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    11821 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    11881 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    11941 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    12001 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
    12061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    12121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    12181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    12241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    12301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    12361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    12421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    12481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    12541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    12601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    12661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    12721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    12781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    12841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    12901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    12961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    13021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    13081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    13141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    13201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    13261 tgccacctga cgtc
//
