LOCUS       V047370                 7840 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047370
VERSION     V047370
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3279
                     /label="TRIM67(NM_001004342)"
                     /note="TRIM67(NM_001004342)"
                     /gene="TRIM67"
     CDS             3286..3312
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3316..3342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3346..3372
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3407..3631
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3677..4105
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4119..4448
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4515..5309
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5483..5604
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5653..5669)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5677..5693
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5701..5731)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5746..5767
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6055..6640)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6811..7671)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7672..7776)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggaggaag agctgaagtg tcccgtgtgc
      961 ggctctctgt ttcgggagcc tatcatcctg ccctgttccc acaatgtctg cctgccttgc
     1021 gctcgcacca tcgcggtgca gaccccggac ggtgagcagc acctgcccca gccgctcctg
     1081 ctttcccggg gatcggggct gcaggcgggc gccgccgccg ctgcctctct ggagcacgac
     1141 gctgcggctg gcccggcctg cggcggtgca ggcgggagtg cagctggcgg cctcggcggc
     1201 ggtgcgggag gtggcggaga ccacgcggac aagctcagct tgtacagcga gacagacagc
     1261 ggctacgggt cctacacccc gagcctcaag tcccccaacg gggttcgcgt gctgcccatg
     1321 gtgcccgcac cacccggctc ctcggctgcg gcggctcggg gtgccgcctg ctcctcgctg
     1381 tcctcgtctt cgagctccat cacgtgcccg cagtgccacc gcagcgcatc cctggaccac
     1441 cgcggcctgc gcggcttcca gcgcaaccgg ctgctcgagg ccatcgtgca gcggtaccag
     1501 cagggccgcg gggccgtgcc ggggacgtct gcagccgcgg cggtggccat ctgccagctg
     1561 tgcgaccgca ccccgccaga gccagcagcc acgctctgcg agcagtgcga cgtcctctac
     1621 tgctctgcct gccagctcaa gtgccatcca tcccggggac ccttcgccaa gcatcgcctg
     1681 gtgcagccgc cgccgccgcc gccgccgccc gccgaggcag cctccgggcc cactggcacc
     1741 gcccagggcg cccccagcgg aggcggcggc tgcaagagcc cgggaggcgc gggggcgggg
     1801 gcgactgggg gcagcacggc ccgcaagttc cccacgtgtc ccgagcatga aatggagaac
     1861 tacagcatgt actgcgtgag ctgtcgaacc ccggtgtgtt atctgtgcct ggaggagggc
     1921 cggcacgcca agcacgaggt gaagccgctg ggggccatgt ggaagcagca caaggcacaa
     1981 ctatctcagg ccttaaatgg agtttcagat aaggcaaagg aagcaaagga gtttctggtt
     2041 cagctaaaga acatattgca gcagatccag gaaaacggac tggactacga agcctgcctc
     2101 gttgctcagt gtgatgccct tgtggatgct ttaactcgtc agaaagccaa gctgctcacc
     2161 aaggtgacta aagagaggga acacaagttg aagatggttt gggaccagat caatcactgc
     2221 acattgaagc tgcgtcagtc caccggactg atggagtact gcctggaggt gatcaaggag
     2281 aacgacccct ccgggttctt acagatctca gatgctctga tcaagcgcgt ccaggtgtct
     2341 caggagcagt gggtcaaagg cgccctggag ccgaaagtgt ctgcggagtt tgatctgact
     2401 ttggacagcg agccgctgct gcaggccatc caccagctgg acttcattca gatgaaatgt
     2461 agggtgccac ccgtccccct actgcagctg gagaaatgct gcacccgtaa caacagcgtc
     2521 acgctggcct ggaggatgcc acccttcacc cacagccccg tggacggcta catcctggag
     2581 ctggacgacg gtgccggggg acagttccgg gaagtgtacg tcggtaagga gactttgtgt
     2641 accatcgacg gtcttcactt caacagcacc tacaacgccc gagtcaaagc tttcaactct
     2701 tctggtgtcg ggccttacag taaaactgtc gtcctgcaga catccgatgt ggcctggttc
     2761 acatttgacc ccaactctgg gcatcgggac atcattttat ccaatgacaa ccagacagcc
     2821 acctgcagca gctatgacga ccgggtggtg ctgggcacag ctgcgttctc caagggcgtg
     2881 cactactggg agctgcacgt ggaccggtac gacaaccacc cagaccccgc cttcggggtg
     2941 gccagggcca gcgtggtcaa ggacatgatg ctgggcaagg atgacaaggc ctgggccatg
     3001 tatgtggaca acaaccgcag ctggttcatg cactgcaact cccacaccaa caggacggaa
     3061 ggtggcgtgt gcaagggggc caccgtgggc gtgctgctgg acctgaataa gcacactctc
     3121 accttcttca tcaacgggca gcagcagggc cccacagcct tcagccacgt ggacggggtc
     3181 ttcatgccag ccctcagcct caaccgcaac gtgcaggtca ccctgcacac aggattggaa
     3241 gtgccgacta acctggggcg gccaaagctg tcaggcaatc tcgagtaccc atacgacgta
     3301 cctgattatg caacttatcc ttatgatgta cctgattatg ctagctatcc ttatgatgtc
     3361 ccagactacg catgagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3421 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3481 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3541 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3601 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3661 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3721 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3781 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3841 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3901 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3961 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4021 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4081 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4201 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4261 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4381 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4441 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4681 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5041 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5281 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5341 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5401 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5461 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5521 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5581 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5641 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5701 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5761 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5821 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5881 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6241 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6481 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     6601 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6661 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6721 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6781 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6841 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6901 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6961 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7021 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7081 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7141 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7201 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7261 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7321 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7381 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7441 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7501 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7561 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7621 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7681 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7741 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7801 tgacgtcgac ggatcgggag atctcccgat cccctatggt
//
