LOCUS       V038620                 8761 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038620
VERSION     V038620
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     regulatory      913..922
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             928..1017
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     CDS             1033..4281
                     /label="UBE3C(NM_014671)"
                     /note="UBE3C(NM_014671)"
                     /gene="UBE3C"
     polyA_signal    4361..4585
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4631..5059
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5073..5402
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5469..6263
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6437..6558
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6607..6623)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6631..6647
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6655..6685)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6700..6721
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7009..7594)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7765..8625)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8626..8730)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagccaccat ggcggcggaa caaaaactaa tatcagagga agatttggaa
      961 cagaaactaa tcagtgaaga agatttagag caaaagttaa tttccgagga ggacttatca
     1021 gcggaaaagc ttatgttcag cttcgaaggc gacttcaaga cgcggcccaa ggtgtccctt
     1081 ggcggcgcga gcaggaagga ggaaaaggct tctcttttac atcgtactca ggaagaaaga
     1141 agaaagagag aggaagaaag gcgaaggttg aaaaatgcaa taattatcca gtcatttatt
     1201 cgaggctata gagacagaaa acagcaatat tccatccaaa gaagtgcatt tgatcgctgt
     1261 gctaccttgt cacagtccgg gggcgctttt cccattgcta atggccccaa ccttaccctt
     1321 ttggtaaggc agcttctgtt tttttacaaa caaaatgaag actcaaaacg tttgatatgg
     1381 ctgtatcaga acttaattaa acacagctct ctgtttgtca agcagttgga tggatctgag
     1441 agacttacat gcttatttca gataaaaaga ttgatgagcc tctgttgcag gttgctgcaa
     1501 aactgtaatg atgacagttt gaatgttgca cttccaatga gaatgcttga agtattttcg
     1561 tctgagaata cttacttgcc tgttttacaa gatgctagct atgtggtgtc agtgattgaa
     1621 caaattttgc actacatgat tcacaatggg tattataggt ctctatattt gttgattaac
     1681 agcaagcttc catcaagtat tgaatattct gatttatctc gagttcctat agcaaaaatt
     1741 ttgctagaga atgttctaaa accattgcac tttacttaca actcctgtcc ggaaggtgcg
     1801 aggcaacaag tttttacagc cttcacagag gagtttctgg cagcaccttt tacagatcag
     1861 atttttcatt tcatcattcc ggcgcttgca gatgcgcaga ccgttttccc ttacgagccc
     1921 tttctgaatg cactgttgtt aatagagagt agatgttcaa gaaagagtgg tggagcaccc
     1981 tggcttttct atttcgtttt aactgttggc gaaaattatt tgggggccct ctctgaggaa
     2041 gggctgctgg tgtatttgcg ggtgctgcag accttcctct ctcagttacc agtctctcct
     2101 gccagcgcga gctgtcacga ctcagccagt gactctgagg aggagagtga agaagccgac
     2161 aagccctcaa gcccggagga tggcagactg tcagtatcat acataacaga ggaatgcctg
     2221 aagaagctgg acacaaagca gcagaccaac accctgctca acctggtgtg gagggactct
     2281 gcgagcgagg aggtcttcac caccatggcc tccgtctgcc acacgctgat ggtgcagcac
     2341 cgcatgatgg tacccaaagt caggcttctc tacagtttag cctttaatgc caggtttctg
     2401 agacatcttt ggtttctaat atcttccatg tcaacacgga tgatcacagg gtctatggta
     2461 ccgttgcttc aggtgatatc caggggttct cctatgtctt ttgaagattc tagtcgaatc
     2521 atcccactct tttatctttt tagctccttg tttagtcatt cactaatttc catacatgat
     2581 aacgaattct tcggtgatcc catagaagtt gtaggtcaaa gacaatcatc aatgatgcct
     2641 tttactttag aagagctgat aatgttgtct cgatgccttc gagatgcatg cctggggatc
     2701 atcaagttgg cttatccaga aaccaagcca gaagttcgag aagaatatat tacagcattt
     2761 cagagtattg gagttactac tagctctgaa atgcaacaat gcatacagat ggaacagaaa
     2821 agatggattc agttatttaa ggttatcacc aatctagtga aaatgttgaa gtccagagac
     2881 acgaggagaa atttttgtcc tccaaaccac tggctgtcag aacaagaaga tattaaagca
     2941 gataaggtca ctcagctcta tgtgccagca tccagacatg tgtggaggtt ccggcggatg
     3001 gggaggatag gcccgctgca gtccaccctg gacgtgggtt tggagtcccc gccgctgtct
     3061 gtgtctgagg aaagacagct tgctgtcctg acagagttgc cttttgtggt tccatttgag
     3121 gaacgagtaa agatctttca gaggttgatt tatgcagata agcaagaagt tcaaggagat
     3181 ggtccatttc tggatggaat taatgtcaca ataagaagaa attacattta tgaagatgct
     3241 tatgacaaac tttctccaga aaatgagcct gatttgaaaa agcggatccg tgtgcacttg
     3301 ctcaatgccc atggcctgga tgaagctggc attgatggtg gtggtatttt cagagagttt
     3361 ttaaatgaac tactgaagtc aggatttaac cccaaccagg ggttctttaa gactactaat
     3421 gaagggcttc tgtaccccaa cccggctgct cagatgcttg tgggagattc ttttgccaga
     3481 cattactact tcctaggcag aatgcttgga aaggctctct atgagaacat gctggtggag
     3541 ctgccctttg caggcttctt tctttccaag ttgcttggaa ccagtgccga cgtggacatt
     3601 caccacctcg cctccctaga ccctgaggtg tataagaatt tgctctttct gaagagctac
     3661 gaagacgatg tggaggagct tgggctgaac ttcactgtgg tgaacaatga cctgggagag
     3721 gcgcaggtag ttgaactaaa attcggtggg aaagacatcc ctgtcaccag cgccaaccgg
     3781 attgcgtaca tccacttggt ggcagactac aggctgaaca ggcagatccg ccagcactgc
     3841 ctggctttcc gccagggcct tgccaatgtc gtcagcctcg agtggctccg aatgtttgat
     3901 cagcaagaaa ttcaggtatt aatttctggt gcacaagttc ccataagcct agaggaccta
     3961 aaatccttta caaactattc aggaggctat tctgcagacc atcctgttat taaggtcttc
     4021 tggagagttg tggaagggtt cactgatgaa gaaaagcgca aactgctgaa gtttgtaaca
     4081 agctgctctc gaccccctct cttggggttt aaggagttgt atcccgcatt ttgtattcac
     4141 aacggaggct ccgaccttga gcggctcccc acagccagca cctgcatgaa cctgctgaag
     4201 ctccccgagt tctatgacga gacacttttg cgaagtaaac ttctctatgc gattgaatgt
     4261 gccgctggct ttgagctgag ctgagaattc tgcagatatc cagcacagtg gcggccgctc
     4321 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4381 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4441 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4501 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4561 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4621 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4681 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4741 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4801 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4861 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4921 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4981 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5041 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5101 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5221 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5281 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5341 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5401 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5461 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5521 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5581 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5641 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5701 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5761 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5821 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5881 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5941 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6001 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6061 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6121 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6181 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6241 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6301 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6361 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6421 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6481 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6541 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6601 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6661 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7441 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     7561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8761 c
//
