LOCUS       V028909                 7816 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028909
VERSION     V028909
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3336
                     /label="USP10(NM_001272075)"
                     /note="USP10(NM_001272075)"
                     /gene="USP10"
     polyA_signal    3383..3607
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3653..4081
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4095..4424
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4491..5285
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5459..5580
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5629..5645)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5653..5669
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5677..5707)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5722..5743
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6031..6616)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6787..7647)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7648..7752)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgccctggt tgccctctcc tggaataggg
      961 cagtatattt ttggagattt tagccctgat gaattcaatc aattctttgt gactcctcga
     1021 tcttcagttg agcttcctcc atacagtgga acagttctgt gtggcacaca ggctgtggat
     1081 aaactacctg atggacaaga atatcagaga attgagtttg gtgtcgatga agtcattgaa
     1141 cccagtgaca ctttgccgag aacccccagc tacagtattt caagcacact gaaccctcag
     1201 gcccctgaat ttattctcgg ttgtacagct tccaaaataa cccctgatgg tatcactaaa
     1261 gaagcaagct atggctccat cgactgccag tacccaggct ctgccctcgc tttggatgga
     1321 agttctaatg tggaggcgga agttttggaa aatgatggtg tctcaggtgg tcttggacaa
     1381 agggagcgta aaaagaagaa aaagcggcca cctggatatt acagctattt gaaagatggt
     1441 ggcgatgata gtatctccac agaagccctg gtcaatggcc atgccaattc agcagtcccg
     1501 aacagtgtca gtgcagagga tgcagaattt atgggtgaca tgcccccgtc agttacgccc
     1561 aggacttgta acagccccca gaactccaca gactctgtca gtgacattgt gcctgacagt
     1621 cctttccccg gagcactcgg cagtgacacc aggactgcag ggcagccaga ggggggcccc
     1681 ggggctgatt ttggtcagtc ctgcttccct gcagaggctg gcagagacac cctgtcaagg
     1741 acagctgggg ctcagccctg cgttggtacc gatactactg aaaaccttgg agttgctaat
     1801 ggacaaatac ttgaatcctc gggtgagggc acagctacca acggggtgga gttgcacacc
     1861 acggaaagca tagacttgga cccaaccaaa cccgagagtg catcacctcc tgctgacggc
     1921 acgggctctg catcaggcac ccttcctgtc agccagccca agtcctgggc cagcctcttt
     1981 catgattcta agccctcttc ctcctcgccg gtggcctatg tggaaactaa gtattcccct
     2041 cccgccatat ctcccctggt ttctgaaaag caggttgaag tcaaagaagg gcttgttccg
     2101 gtttcagagg atcctgtagc cataaagatt gcagagttgc tggagaatgt aaccctaatc
     2161 cataaaccag tgtcgttgca accccgtggg ctgatcaata aagggaactg gtgctacatt
     2221 aatgctacac tgcaggcatt ggttgcttgc ccgccgatgt accacctgat gaagttcatt
     2281 cctctgtatt ccaaagtgca aaggccttgt acgtcaacac ccatgataga cagctttgtt
     2341 cggctaatga atgagttcac taatatgcca gtacctccaa aaccccgaca agctcttgga
     2401 gataaaatcg tgagggatat tcgccctgga gctgcctttg agcccacata tatttacaga
     2461 ctcctgacag ttaacaagtc aagcctgtct gaaaagggtc gacaagaaga tgctgaggaa
     2521 tacttaggct tcattctaaa tggacttcat gaggaaatgt tgaacctaaa gaagcttctc
     2581 tcaccaagta atgaaaaact tacgatttcc aacggcccca aaaaccactc ggtcaatgaa
     2641 gaagagcagg aagaacaagg tgaaggaagc gaggatgaat gggaacaagt gggcccccgg
     2701 aacaagactt ccgtcacccg ccaggcggat tttgttcaga ctccaatcac cggcattttt
     2761 ggtggacaca tcaggtctgt ggtttaccag cagagttcaa aagaatctgc cactttgcag
     2821 ccatttttca cgttgcagtt ggatatccag tcagacaaga tacgcacagt ccaggatgca
     2881 ctggagagct tggtggcaag agaatctgtc caaggttata ccacaaaaac caaacaagag
     2941 gttgagataa gtcgaagagt gactctggaa aaactccctc ctgtcctcgt gctgcacctg
     3001 aaacgattcg tttatgagaa gactggtggg tgccagaagc ttatcaaaaa tattgaatat
     3061 cctgtggact tggaaattag taaagaactg ctttctccag gggttaaaaa taagaatttt
     3121 aaatgccacc gaacctatcg gctctttgca gtggtctacc atcacggcaa cagtgcgacg
     3181 ggcggccatt acactacaga cgtcttccag atcggtctga atggctggct gcgcatcgat
     3241 gaccagacag tcaaggtgat caaccagtac caggtggtga aaccaactgc tgaacgcaca
     3301 gcctacctcc tgtattaccg ccgagtggac ctgctgtaac tcgagtctag agggcccgtt
     3361 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3421 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3481 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3541 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3601 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     3661 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3721 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3781 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3841 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3901 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3961 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4021 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4081 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4141 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4201 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4261 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4321 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4381 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4441 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4501 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4561 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4621 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     4681 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4741 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4801 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4861 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4921 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4981 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5041 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5101 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5161 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5221 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5281 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5341 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5401 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5461 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5521 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5581 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5641 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5701 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5761 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5821 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5881 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5941 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6541 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     7801 cccgatcccc tatggt
//
