LOCUS V026046 6566 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026046 VERSION V026046 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2056 /label="YBX3(NM_003651)" /note="YBX3(NM_003651)" /gene="YBX3" CDS 2063..2128 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2166..2390 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2436..2864 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2878..3207 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3274..4068 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4242..4363 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4412..4428) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4436..4452 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4460..4490) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4505..4526 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4814..5399) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5570..6430) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6431..6535) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgagtgagg cgggcgaggc 961 caccaccacc accaccacca ccctcccgca ggctccgacg gaggcggccg ccgcggctcc 1021 ccaggacccc gcgcccaaga gcccggtggg cagcggtgcg ccccaggccg cggccccggc 1081 gcccgccgcc cacgtcgcag gaaaccccgg tggggacgcg gcccccgcag ccacgggcac 1141 cgcggccgcc gcctctttag ccaccgccgc cggcagcgaa gacgcggaga aaaaagttct 1201 cgccaccaaa gtccttggca ctgtcaaatg gttcaacgtc agaaatggat atggatttat 1261 aaatcgaaat gacaccaaag aagatgtatt tgtacatcag actgccatca agaagaataa 1321 cccacggaaa tatctgcgca gtgtaggaga tggagaaact gtagagtttg atgtggttga 1381 aggagagaag ggtgcagaag ctgccaatgt gactggcccg gatggagttc ctgtggaagg 1441 gagtcgttac gctgcagatc ggcgccgtta cagacgtggc tactatggaa ggcgccgtgg 1501 ccctccccgg aattacgctg gggaggagga ggaggaaggg agcggcagca gtgaaggatt 1561 tgacccccct gccactgata ggcagttctc tggggcccgg aatcagctgc gccgccccca 1621 gtatcgccct cagtaccggc agcggcggtt cccgccttac cacgtgggac agacctttga 1681 ccgtcgctca cgggtcttac cccatcccaa cagaatacag gctggtgaga ttggagagat 1741 gaaggatgga gtcccagagg gagcacaact tcagggaccg gttcatcgaa atccaactta 1801 ccgcccaagg taccgtagca ggggacctcc tcgcccacga cctgccccag cagttggaga 1861 ggctgaagat aaagaaaatc agcaagccac cagtggtcca aaccagccgt ctgttcgccg 1921 tggataccgg cgtccctaca attaccggcg tcgcccgcgt cctcctaacg ctccttcaca 1981 agatggcaaa gaggccaagg caggtgaagc accaactgag aaccctgctc cacccaccca 2041 gcagagcagt gctgagctcg aggactacaa agaccatgac ggtgattata aagatcatga 2101 catcgactac aaggatgacg atgacaagta gtgagggccc gtttaaaccc gctgatcagc 2161 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 2221 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 2281 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 2341 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 2401 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 2461 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 2521 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 2581 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 2641 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 2701 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 2761 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 2821 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 2881 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2941 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 3001 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 3061 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 3121 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 3181 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga 3241 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 3301 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 3361 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 3421 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg 3481 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 3541 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 3601 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 3661 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 3721 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 3781 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc 3841 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 3901 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 3961 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 4021 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 4081 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 4141 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 4201 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt 4261 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 4321 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt 4381 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 4441 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 4501 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 4561 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 4621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 4681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 4741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 4801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 4861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 4921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 4981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 5041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 5101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 5161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 5221 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 5281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 5341 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 5401 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 5461 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 5521 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 5581 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 5641 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 5701 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 5761 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 5821 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 5881 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 5941 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 6001 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 6061 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 6121 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 6181 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 6241 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 6301 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 6361 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 6421 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 6481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 6541 ttccccgaaa agtgccacct gacgtc //