LOCUS V022468 6062 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022468 VERSION V022468 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..1501 /label="Ykt6(NM_019661)" /note="Ykt6(NM_019661)" /gene="Ykt6" CDS 1508..1534 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1538..1564 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1568..1594 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 1629..1853 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1899..2327 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2341..2670 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2737..3531 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3705..3826 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3875..3891) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3899..3915 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3923..3953) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3968..3989 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4277..4862) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5033..5893) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5894..5998) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg aagctgtaca gccttagtgt cctttacaaa ggcgatccga aagcggtgct 961 gctcaaggcc gcgtacgacg tgtcttcctt tagtttcttc cagaggtcca gtgtgcagga 1021 attcatgacc tttacaagtc aactgattgt ggaacgctcg gggaaaggca gcagagcttc 1081 tgtcaaagaa caagagtatc tttgccatgt ctacgtccgg agcgacagtc tggcaggtgt 1141 ggtcattgct gacagcgaat acccttccag agtggccttc accttgctgg agaaggtact 1201 agatgaattc tccaagcagg tggatagaat agactggcca gtaggatccc cggctacaat 1261 ccagtacaca ggcctggacg atcaccttag taaataccag aacccccgag aagctgatcc 1321 catgagtaaa gtgcaggccg aactggacga gaccaaaatc attctgcaca acaccatgga 1381 gtctttgtta gagcgaggcg agaagcttga tgacctggta tccaagtcag aagtgctggg 1441 aacacagtcg aaagccttct ataaaactgc ccggaaacaa aactcttgct gtgcaatcat 1501 gctcgagtac ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta 1561 tgctagctat ccttatgatg tcccagacta cgcatgaggg cccgtttaaa cccgctgatc 1621 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 1681 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 1741 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 1801 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 1861 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 1921 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 1981 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 2041 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 2101 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 2161 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 2221 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 2281 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 2341 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 2401 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 2461 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 2521 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 2581 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 2641 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 2701 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 2761 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 2821 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 2881 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 2941 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 3001 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 3061 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 3121 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 3181 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 3241 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 3301 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 3361 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 3421 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 3481 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 3541 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 3601 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 3661 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 3721 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 3781 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 3841 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 3901 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 3961 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 4021 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 4081 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4681 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 4741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4801 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 4861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 4921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 4981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 5041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 5101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 5161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 5221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 5281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 5341 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 5401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 5461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 5521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 5581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 5641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 5701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 5761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 5821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 5881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 5941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 6001 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg 6061 gt //