LOCUS       V027678                 7880 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027678
VERSION     V027678
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             941..3370
                     /label="ZC3H11A(NM_014827)"
                     /note="ZC3H11A(NM_014827)"
                     /gene="ZC3H11A"
     CDS             3377..3442
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3480..3704
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3750..4178
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4192..4521
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4588..5382
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5556..5677
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5726..5742)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5750..5766
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5774..5804)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5819..5840
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6128..6713)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6884..7744)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7745..7849)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgcctaatc aaggagaaga
      961 ctgctatttt tttttctatt ccacatgtac caaaggtgac agctgcccat tccgtcactg
     1021 tgaagctgca ataggaaatg aaactgtttg cacattatgg caagaagggc gctgttttcg
     1081 acaggtgtgc aggtttcggc acatggagat tgataaaaaa cgcagtgaaa ttccttgtta
     1141 ttgggaaaat cagccaacag gatgtcaaaa attaaactgc gctttccatc acaatagagg
     1201 acgatatgtt gatggccttt tcctacctcc gagcaaaact gtgttgccca ctgtgcctga
     1261 gtcaccagaa gaggaagtga aggctagcca actttcagtt cagcagaaca aattgtctgt
     1321 ccagtccaat ccttcccctc agctgcggag cgttatgaaa gtagaaagtt ccgaaaatgt
     1381 tcctagcccc acgcatccac cagttgtaat taatgctgca gatgatgatg aagatgatga
     1441 tgatcagttt tctgaggaag gtgatgaaac caaaacacct accctgcaac caactcctga
     1501 agttcacaat ggattacgag tgacttctgt ccggaaacct gcagtcaata taaagcaagg
     1561 tgaatgtttg aattttggaa taaaaactct tgaggaaatt aagtcaaaga aaatgaagga
     1621 aaaatctaag aagcaaggtg agggttcttc aggagtttcc agtcttttac tccaccctga
     1681 gcccgttcca ggtcctgaaa aagaaaatgt caggactgtg gtgaggacag taactctctc
     1741 caccaaacaa ggagaagaac ccttggttag attgagtctt actgagagac tggggaaacg
     1801 aaaattttca gcaggcggtg acagtgatcc tccattaaag cgtagcctgg cacagaggct
     1861 agggaagaaa gttgaagctc cagaaactaa cattgacaaa acaccaaaga aagctcaagt
     1921 ttccaagtct cttaaggagc gattaggcat gtcagctgat ccagataatg aggatgcaac
     1981 agataaagtt aataaagttg gtgagatcca tgtgaagaca ttagaagaaa ttcttcttga
     2041 aagagccagt cagaaacgtg gagaattgca aactaaactc aagacagaag gaccttcaaa
     2101 aactgatgat tctacttcag gagcaagaag ctcctccact atccgtatca aaaccttctc
     2161 tgaggtcctg gctgaaaaaa aacatcggca gcaggaagca gagagacaaa aaagcaaaaa
     2221 ggatacaact tgcatcaagc taaagattga tagtgaaatt aaaaaaacag tagttttgcc
     2281 acccattgtt gccagcagag gacaatcaga ggagcctgca ggtaaaacaa agtctatgca
     2341 ggaggtgcac atcaagacgc tggaagaaat taaactggag aaggcactga gggtgcagca
     2401 gagctctgag agcagcacca gctccccgtc tcaacacgag gccactccag gggcaaggcg
     2461 gctgctgcga atcaccaaaa gaacagggat gaaagaagag aagaaccttc aggaaggaaa
     2521 tgaagttgat tctcagagca gtattagaac agaagctaaa gaggcttcag gtgagaccac
     2581 aggagttgac atcactaaaa ttcaagtcaa gagatgtgag accatgagag agaagcacat
     2641 gcagaaacag caggagaggg aaaaatcagt cttgacacct cttcggggag atgtagcctc
     2701 ttgcaatacc caagtggcag agaaaccagt gctcactgct gtgccaggaa tcacacggca
     2761 cctgaccaag cggcttccca caaagtcatc ccagaaggtg gaggtagaaa cctcagggat
     2821 tggagactca ttattgaatg tgaaatgtgc agcacagacc ttggaaaaaa ggggtaaagc
     2881 taaacccaaa gtgaacgtga agccatctgt ggttaaagtt gtgtcatccc ccaaattggc
     2941 cccaaaacgt aaggcagtgg agatgcacgc tgctgtcatt gccgctgtga agccactcag
     3001 ctccagcagt gtcctacagg aacccccagc caaaaaggca gctgtggctg ttgtcccgct
     3061 tgtctctgag gacaaatcag tcactgtgcc tgaagcagaa aatcctagag acagtcttgt
     3121 gctgcctcca acccagtcct cttcagattc ctcacccccg gaggtgtctg gcccttcctc
     3181 atcccaaatg agcatgaaaa ctcgccgact cagctctgcc tcaacaggaa agcccccact
     3241 ctctgtggag gatgattttg agaaactaat atgggagatt tcaggaggca aattggaagc
     3301 tgagattgac ctggatcctg ggaaagatga agatgacctt ctgcttgagc tatcagaaat
     3361 gattgatagc ctcgaggact acaaagacca tgacggtgat tataaagatc atgacatcga
     3421 ctacaaggat gacgatgaca agtagtgagg gcccgtttaa acccgctgat cagcctcgac
     3481 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3541 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3601 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3661 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3721 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3781 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3841 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3901 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3961 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4021 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4081 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4141 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4201 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4261 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4321 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4381 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4441 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     4501 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     4561 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     4621 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4681 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4741 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4801 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     4861 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     4921 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4981 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5041 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5101 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5161 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5221 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5281 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5341 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5401 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     5461 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5521 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     5581 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5641 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     5701 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5761 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5821 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5881 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5941 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6001 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6061 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6121 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6181 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6241 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6301 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6361 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6421 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6481 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6541 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6601 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6661 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7201 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7261 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7321 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7381 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7441 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7501 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7561 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7621 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7681 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7741 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7801 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7861 gaaaagtgcc acctgacgtc
//
