LOCUS       V036161                 8940 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036161
VERSION     V036161
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             932..4855
                     /label="CNTNAP4(NM_033401)"
                     /note="CNTNAP4(NM_033401)"
                     /gene="CNTNAP4"
     CDS             4862..4891
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4907..4924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     polyA_signal    4953..5177
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5223..5651
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5665..5995
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        6043..6090
                     /note="synthetic bacterial promoter&nbsp;"
                     /label="EM7 promoter"
     CDS             6109..6483
                     /codon_start=1
                     /gene="Sh&nbsp;ble from&nbsp;Streptoalloteichus
                     hindustanus"
                     /note="confers resistance to bleomycin, phleomycin, and
                     Zeocin™"
                     /product="antibiotic-binding protein"
                     /transl_table=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD*"
                     /label="BleoR"
     polyA_signal    6613..6734
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6783..6799)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6807..6823
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6831..6861)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6876..6897
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7185..7773)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7944..8804)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8805..8909)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccgccac catgggatct gtcacgggag ctgtcctcaa
      961 gacgctactt ctgttatcta ctcaaaattg gaacagagtc gaagctggga attcctatga
     1021 ctgtgatgat cctcttgtgt ctgccttgcc tcaggcatcc ttcagcagtt cttccgagct
     1081 ctccagcagt catggtcctg gatttgcaag gctgaataga agagatggag ctggtggctg
     1141 gtctccactt gtgtctaaca aataccagtg gttgcagatt gaccttggag agagaatgga
     1201 ggtcaccgct gtggccactc aagggggata tggtagctcc aactgggtga ccagctacct
     1261 cctgatgttc agtgatagtg gctggaactg gaaacaatat cgccaagagg acagcatctg
     1321 gggtttttca ggaaatgcaa atgcagacag tgttgtgtac tatagactcc agccttctat
     1381 caaagccaga tttctgcgct tcatcccttt ggaatggaac cccaagggca gaattggaat
     1441 gcgaatcgaa gtgttcggat gtgcatacag atcagaagtg gttgatcttg atggaaaaag
     1501 ttcccttctc tacagatttg atcaaaaatc cctgagccca ataaaagaca ttatttcttt
     1561 gaaattcaaa accatgcaga gtgatgggat tctactccac agggaagggc caaatggaga
     1621 tcacatcaca ctgcaattaa gaagagcaag actcttttta cttattaatt caggtgaagc
     1681 taaactgcct tccacttcca ccctggtcaa tctcaccctg ggcagcctgc tagatgatca
     1741 gcattggcat tcagtgctca tccagcgttt gggcaaacaa gtcaacttca cagtggacga
     1801 acacaggcat catttccatg cacggggaga attcaatctc atgaatcttg attatgagat
     1861 cagctttgga gggattccag cacctggaaa atcagtgtca ttcccacata gaaattttca
     1921 tggatgttta gaaaatctct attataatgg agtggatatc attgatttgg ccaagcagca
     1981 aaaaccacag atcattgcta tgggaaatgt gtcattttct tgttcacaac cacaatctat
     2041 gcccgtgact tttctgagct ccaggagtta tttagcactg ccagacttct ctggagagga
     2101 ggaggtttct gccacttttc aatttcgaac ttggaataag gcagggcttc tgctgttcag
     2161 tgaacttcag ctgatttcag ggggtatcct cctctttctg agtgatggaa aacttaagtc
     2221 gaatctctac cagccaggaa aattacccag tgacatcaca gcaggtgtcg aattaaatga
     2281 tgggcagtgg cattctgtct ctttatctgc taaaaagaat cacttgagtg tggcggtgga
     2341 cggccagatg gcttctgctg ctcctctgct ggggcctgag cagatttatt cgggtggcac
     2401 ctattatttt ggaggttgtc ctgacaaaag ctttggatcc aaatgtaaaa gtccacttgg
     2461 tggatttcag ggatgtatga ggctcatttc tatcagcggc aaagtggtag atctgatttc
     2521 agttcagcag gggtcccttg ggaacttcag tgaccttcag atagactcat gtggcatctc
     2581 agacaggtgt ttgcccaact attgtgaaca cggtggggag tgttcccagt cctggagcac
     2641 ctttcattgt aactgtacca acactggtta cagaggagct acttgccata actctatcta
     2701 tgagcagtca tgtgaagcct ataagcacag aggaaatact tcagggtttt actatataga
     2761 ttcagatgga agtggtcccc tggaaccatt tcttctatat tgcaatatga ccgaaactgc
     2821 atggaccatc atacagcaca acggctctga cttaacaaga gtcagaaata ctaatccaga
     2881 gaacccatat gctgggtttt tcgagtatgt ggccagcatg gagcaacttc aggccactat
     2941 taaccgtgca gagcactgtg aacaggagtt tacttattac tgcaagaagt cacggctggt
     3001 caataagcaa gatggaaccc ctctgagttg gtgggtagga agaaccaatg aaacgcaaac
     3061 ctactgggga ggttcttcgc ctgatcttca aaaatgtact tgtggattag agggaaactg
     3121 cattgattct cagtattact gcaattgtga tgctgaccgg aatgaatgga ccaatgacac
     3181 tggattgctt gcttataaag aacatcttcc agtaactaag atcgtgatta cagacacagg
     3241 ccgactgcat tcagaagcag cttataaact ggggcctctg ctctgccagg gagacagatc
     3301 attttggaat tcagcttcct ttgataccga ggcttcatat cttcattttc ctaccttcca
     3361 cggagaactt agcgcggatg tatctttctt ttttaagaca acagcttcat ctggggtatt
     3421 tttagagaac ttggggattg ctgattttat acggatagag cttcgctctc cgacagtagt
     3481 gactttttca tttgatgtgg ggaatgggcc ttttgaaatc tcagtgcagt cacccaccca
     3541 cttcaacgac aaccagtggc accatgtgag ggttgaaagg aacatgaagg aggcctccct
     3601 tcaagtggat cagctgacac caaagacaca gcccgccccc gctgatgggc atgtcctgtt
     3661 acagctcaac agtcagctct tcgtgggtgg aacggccacc agacagagag gctttctggg
     3721 ctgcattcgg tctctgcagt tgaatgggat gaccctggat ttggaagaaa gagcccaggt
     3781 gactccagaa gtgcagccag gttgtagggg acattgcagc agctatggga agttatgccg
     3841 caatggaggg aaatgcagag aaagacccat tgggttcttt tgtgactgca ctttctctgc
     3901 atacacaggg ccattctgct caaatgagat ttctgcatat tttggatctg gctcatccgt
     3961 gatatacaat tttcaagaaa attatctttt aagtaaaaac tccagctccc acgctgcttc
     4021 atttcatggt gatatgaagc tgagcagaga aatgatcaaa tttagtttcc gaacaacacg
     4081 aacaccaagc ttgctgcttt ttgtgagctc cttttacaaa gaataccttt ctgtgatcat
     4141 tgccaaaaat ggaagtttgc agatcaggta caagttaaat aaatatcaag agcctgatgt
     4201 tgttaacttt gattttaaaa acatggctga tggacaactt caccacataa tgattaacag
     4261 agaagaagga gtggtcttta tagagattga cgataataga aggagacaag ttcacctgtc
     4321 atcaggcaca gaattcagtg cagtcaaatc tctggtattg ggcaggattt tagaacacag
     4381 tgatgtggac caggatactg cactggcagg tgcgcagggc ttcacaggct gcctctctgc
     4441 agtgcagctc agccacgtgg cccctctgaa ggcagctctg caccccagcc acccagaccc
     4501 tgtcactgtt acaggacacg tgactgagtc cagctgtatg gcccagcctg gcactgatgc
     4561 cacatcaagg gaaaggacac actcgtttgc agatcattct ggaacaatag atgacagaga
     4621 gccccttgct aatgcaatca aaagtgactc tgcagtaatt ggaggtctga tagctgttgt
     4681 gatttttatc ttgctttgca tcactgccat agctgttcgc atttatcagc agaaaaggtt
     4741 atataaaaga agtgaggcaa aaaggtcaga gaatgtagac agtgctgagg ctgttctgaa
     4801 aagtgagctt aatatacaaa atgcagtcaa tgaaaatcag aaagagtact tcttcctcga
     4861 ggaacaaaaa ctcatctcag aagaggatct gaatatgaat accggtcatc atcaccatca
     4921 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4981 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5041 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5101 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5161 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5221 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5281 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5341 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
     5401 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5461 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5521 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5581 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5641 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5701 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5761 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5821 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5881 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5941 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6001 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     6061 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     6121 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     6181 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     6241 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     6301 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     6361 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     6421 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     6481 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6541 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6601 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6661 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6721 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6781 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6841 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6961 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7021 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7081 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7141 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7201 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7261 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7321 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     7381 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7441 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7501 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7561 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7621 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7681 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7741 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7801 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7861 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7921 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7981 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8041 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8101 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8161 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8221 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8281 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8341 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8401 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8461 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8521 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8581 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8641 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8701 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8761 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8821 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8881 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
