pFA6a-3HA-kanMX6 载体 (V010312#)

载体基本信息
载体名称 pFA6a-3HA-kanMX6 载体抗性 Ampicillin
载体长度 4248 bp 载体类型 Yeast Plasmids
载体来源 Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 拷贝数 High copy number

pFA6a-3HA-kanMX6 载体载体图谱

pFA6a-3HA-kanMX64248 bp6001200180024003000360042003xHAADH1 terminatorkanMXT7 promoteroriAmpRAmpR promoter

pFA6a-3HA-kanMX6 载体载体序列

LOCUS       Exported                4248 bp ds-DNA    circular SYN 24-12-2013
DEFINITION  Plasmid with a kanMX marker for adding a C-terminal triple-HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-3HA-kanMX6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     CDS             69..158
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /note="3xHA"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      191..378
                     /note="ADH1 terminator"
     gene            425..1781
                     /note="kanMX"
                     /note="yeast selectable marker conferring kanamycin 
                     resistance"
     promoter        425..768
                     /note="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     CDS             769..1578
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1584..1781
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1886..1904)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2162..2750)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2921..3781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3782..3886)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        4232..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"

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