载体基本信息 | |||
载体名称 | pYUB28b | 载体长度 | 4921 bp |
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载体类型 | Cloning vector | 载体来源 | Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. |
pYUB28b 载体载体图谱
pYUB28b 载体载体序列
LOCUS 40924_48288 4921 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pYUB28b, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4921) AUTHORS Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. TITLE Metabolic Engineering of Cofactor F(420) Production in Mycobacterium smegmatis JOURNAL PLoS ONE 5 (12), E15803 (2010) PUBMED 21209917 REFERENCE 2 (bases 1 to 4921) AUTHORS Bashiri G, Baker EN. TITLE Direct Submission JOURNAL Submitted (08-SEP-2010) School of Biological Sciences, The University of Auckland, 3a Symonds St., Auckland, Auckland 1010, New Zealand REFERENCE 3 (bases 1 to 4921) TITLE Direct Submission REFERENCE 4 (bases 1 to 4921) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; date: "2010"; volume: "5"; issue: "12"; pages: "E15803" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (08-SEP-2010) School of Biological Sciences, The University of Auckland, 3a Symonds St., Auckland, Auckland 1010, New Zealand" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4921 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 61..79 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 80..104 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 119..141 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 160..177 /label=6xHis /note="6xHis affinity tag" CDS 187..204 /label=thrombin site /note="thrombin recognition and cleavage site" CDS 208..240 /label=T7 tag (gene 10 leader) /note="leader peptide from bacteriophage T7 gene 10" CDS 289..306 /label=6xHis /note="6xHis affinity tag" terminator 373..420 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 457..885 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" rep_origin complement(3001..3589) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS join(3928..4921,1..2) /gene="hyg" /label=hyg /note="Hygromycin-B 7''-O-kinase from Streptomyces hygroscopicus. Accession#: P09979"
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