pYUB28b 载体 (V001865#)

载体基本信息
载体名称 pYUB28b 载体长度 4921 bp
载体类型 Cloning vector 载体来源 Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN.

pYUB28b 载体载体图谱

pYUB28b4921 bp6001200180024003000360042004800T7 promoterlac operatorRBS6xHisthrombin siteT7 tag (gene 10 leader)6xHisT7 terminatorf1 oriorihyg

pYUB28b 载体载体序列

LOCUS       40924_48288        4921 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYUB28b, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN.
  TITLE     Metabolic Engineering of Cofactor F(420) Production in Mycobacterium
            smegmatis
  JOURNAL   PLoS ONE 5 (12), E15803 (2010)
  PUBMED    21209917
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Bashiri G, Baker EN.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2010) School of Biological Sciences, The 
            University of Auckland, 3a Symonds St., Auckland, Auckland 1010, New
            Zealand
REFERENCE   3  (bases 1 to 4921)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2010"; volume: "5"; issue: "12"; pages: "E15803"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-SEP-2010) School of Biological Sciences, The University of 
            Auckland, 3a Symonds St., Auckland, Auckland 1010, New Zealand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             160..177
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             187..204
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             208..240
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             289..306
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      373..420
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      457..885
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             join(3928..4921,1..2)
                     /gene="hyg"
                     /label=hyg
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces 
                     hygroscopicus. Accession#: P09979"

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