重组大肠杆菌ACNB蛋白

大肠杆菌

大肠杆菌

Met 1 - Val 865

A DNA sequence encoding the E.Coli (strain K12) acnB (P36683) (Met 1-Val 865) was expressed and purified.

> 97 % as determined by SDS-PAGE

The recombinant E.Coli acnB consisting of 865 amino acids and has a calculated molecular mass of 93.5 kDa. It migrates as an approximately 95 kDa band in SDS-PAGE under reducing conditions.

ESCHERICHIA COLI; YACI; ACNB; YACJ

Aconitate hydratase B

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Also catalyzes the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnB functions as a RNA-binding regulatory protein. During oxidative stress inactive AcnB apo-enzyme without iron sulfur clusters binds the acnB mRNA 3' UTRs (untranslated regions), stabilizes acnB mRNA and increases AcnB synthesis, thus mediating a post-transcriptional positive autoregulatory switch. AcnB also decreases the stability of the sodA transcript.

Lyophilized from sterile 40mM Tris, 1mM DTT, pH 8.2
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

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Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.