重组大肠杆菌ACNB蛋白

产品详情

  • 产品名称
    重组大肠杆菌ACNB蛋白
  • 蛋白种属
    大肠杆菌
  • 表达宿主
    大肠杆菌
  • Unitprot ID
  • 克隆构建
    A DNA sequence encoding the E.Coli (strain K12) acnB (P36683) (Met 1-Val 865) was expressed and purified.
  • 蛋白纯度
    > 97 % as determined by SDS-PAGE
  • 分子量
    The recombinant E.Coli acnB consisting of 865 amino acids and has a calculated molecular mass of 93.5 kDa. It migrates as an approximately 95 kDa band in SDS-PAGE under reducing conditions.
  • 蛋白别名
    ESCHERICHIA COLI; YACI; ACNB; YACJ
  • 蛋白全名
    Aconitate hydratase B
  • 蛋白描述
    Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Also catalyzes the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnB functions as a RNA-binding regulatory protein. During oxidative stress inactive AcnB apo-enzyme without iron sulfur clusters binds the acnB mRNA 3' UTRs (untranslated regions), stabilizes acnB mRNA and increases AcnB synthesis, thus mediating a post-transcriptional positive autoregulatory switch. AcnB also decreases the stability of the sodA transcript.
  • 发货形式
    Lyophilized from sterile 40mM Tris, 1mM DTT, pH 8.2
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    Please contact us for more information.
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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