- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:WB, IHC-P
- 特异性:Mouse Carbonic Anhydrase IV / Car4 / CA4
产品详情
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产品名称
Anti-Carbonic Anhydrase IV / Car4 / CA4 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Rabbit polyclonal to Carbonic Anhydrase IV / Car4 / CA4
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抗体应用
WB, IHC-P
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应用推荐
WB: 2-10 μg/ml
IHC-P: 0.1-1 μg/ml
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特异性
Mouse Carbonic Anhydrase IV / Car4 / CA4
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蛋白别名
Ca4, CA-IV, Car4, Carbonic Anhydrase IV, Carbonic Anhydrase IV, CAIV, Car4, RP17, RP23-167D6.1, AW456718, CA IV
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制备方法
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase IV / Car4 / CA4 (rh Carbonic Anhydrase IV / Car4 / CA4; NP_031633.1; Met1-Ser277). Carbonic Anhydrase IV / Car4 / CA4 specific IgG was purified by Mouse Carbonic Anhydrase IV / Car4 / CA4 affinity chromatography.
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组分
0.2 μm filtered solution in PBS
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储存方法
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
背景介绍
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.
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参考文献
- Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
- Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20.
- Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.