- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC-P
- 特异性:Human CTSZ / CTSX / Cathepsin Z
产品详情
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产品名称
Anti-CTSZ / CTSX / Cathepsin Z antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Rabbit polyclonal to CTSZ / CTSX / Cathepsin Z
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抗体应用
ELISA, IHC-P
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应用推荐
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CTSZ / CTSX / Cathepsin Z. The detection limit for Human CTSZ / CTSX / Cathepsin Z is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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特异性
Human CTSZ / CTSX / Cathepsin Z
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蛋白别名
AI787083, AU019819, Cathepsin X, Cathepsin X, Cathepsin Z, Cathepsin Z, CTSX, CTSX, Ctsz, CTSZ, D2Wsu143e, FLJ17088, RP23-59M10.3, CTSX, CTSX, AI787083, AU019819, D2Wsu143e
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制备方法
Produced in rabbits immunized with purified, recombinant Human CTSZ / CTSX / Cathepsin Z (rh CTSZ / CTSX / Cathepsin Z; Q9UBR2; Met1-Val303). CTSZ / CTSX / Cathepsin Z specific IgG was purified by Human CTSZ / CTSX / Cathepsin Z affinity chromatography.
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组分
0.2 μm filtered solution in PBS
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储存方法
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
背景介绍
Cathepsin Z (CTSZ), also known as Cathepsin X or CATX, belongs to the C1 family of lysosomal cysteine proteases. Its gene structure and activity properties show several unique features that distinguish it clearly from other human cysteine proteases. It has a very short pro-region that shows no similarity to those of other cathepsins and a three-residue insertion motif that forms a characteristic ‘mini loop’. Cathepsin Z exhibits mono- and di-peptidase activity at its C-terminus, and in contrast to cathepsin B, it does not act as an endopeptidase. It is restricted to the cells of theimmune system, predominantly monocytes, macrophages and dendritic cells. Cathepsin Z is widely expressed in human tissues, suggesting that this enzyme could be involved in the normal intracellular protein degradation taking place in all cell types. It is capable to cleave regulatory motifs at C-terminus affecting the function of targeted molecules. Cathepsin X may regulate also the maturation of dendritic cells, a process, which is crucial in the initiation of adaptive immunity. Furthermore, higher levels of Cathepsin Z are also found in tumour and immune cells of prostate and gastric carcinomas and inmacrophages of gastric mucosa, especially after infection by Helicobacter pylori. Cathepsin Z is also ubiquitously distributed in cancer cell lines and in primary tumors from different sources, suggesting that this enzyme may participate in tumor progression.
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参考文献
- Santamara I, et al. (1998) Cathepsin Z, a novel human cysteine proteinase with a short propeptide domain and a unique chromosomal location. J Biol Chem. 273(27): 16816-23.
- Kos J, et al. (2009) The role of cathepsin X in cell signaling. Cell Adh Migr. 3(2): 164-6.
- Sevenich L, et al. (2010) Synergistic antitumor effects of combined cathepsin B and cathepsin Z deficiencies on breast cancer progression and metastasis in mice. Proc Natl Acad Sci U S A. 107(6): 2497-502.