- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IHC-P, IP
- 特异性:Human ECH1
产品详情
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产品名称
Anti-ECH1 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Rabbit polyclonal to ECH1
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抗体应用
ELISA, WB, IHC-P, IP
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应用推荐
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ECH1. The detection limit for Human ECH1 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/mL
IP: 0.5-2 μg/mg of lysate
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特异性
Human ECH1
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蛋白别名
HPXEL, AA617331, enoyl CoA hydratase 1, peroxisomal, ECH1, HPXEL, delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase, mitochondrial, delta3, 5-delta2, 4-dienoyl-CoA isomerase, enoyl Coenzyme A hydratase 1, peroxisomal, peroxisomal enoyl-CoA hydratase 1, enoyl coenzyme A hydratase 1, peroxisomal, Ech1, AA617331, delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase, mitochondrial, dienoyl-CoA isomerase, peroxisomal/mitochondrial dienoyl-CoA isomerase
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制备方法
Produced in rabbits immunized with purified, recombinant Human ECH1 (rh ECH1; Q13011; Thr34-Leu328). ECH1 specific IgG was purified by Human ECH1 affinity chromatography.
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组分
0.2 μm filtered solution in PBS
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储存方法
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
背景介绍
ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
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参考文献
- Kovalyov LI, et al. (2006) Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry Mosc. 71(4): 448-53.
- Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 127(3):635-48.
- FitzPatrick DR, et al. (1995) Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27(3):457-66.