Anti-Green fluorescent protein / GFP antibody

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  • 抗体类型:单克隆
  • 抗体来源:兔
  • 抗体应用:ELISA, WB, IP
  • 特异性:Aequorea victoria GFP

产品详情

  • 产品名称
    Anti-Green fluorescent protein / GFP antibody
  • 抗体类型
    单克隆
  • 抗体来源
    兔
  • 抗体亚型
    兔IgG
  • 抗体描述
    Rabbit monoclonal to Green fluorescent protein / GFP
  • 抗体应用
    ELISA, WB, IP
  • 应用推荐

    WB: 1/1000-1/5000

    ELISA: 1/10000-1/20000

    This antibody can be used at 1/10000-1/20000 dilution with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is approximately 0.0049 ng/well.

    IP: 1-4 μl/mg of lysate

  • 特异性
    Aequorea victoria GFP
  • 制备方法
    This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (AAB65663; Ser2-Lys238).
  • 组分
    0.2 μm filtered solution in PBS with 5% trehalose
  • 储存方法
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • 背景介绍
    The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
  • 参考文献
    • Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637.
    • Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.
    • Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067.
    • Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028.
    • Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.
    • Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
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