- 抗体类型:单克隆
- 抗体来源:小鼠
- 抗体应用:WB, IHC-P, ICC/IF
- 特异性:Human, Rat, Mouse
产品详情
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产品名称
Anti-Hexokinase I (7A7) Mouse antibody
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抗体类型
单克隆
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抗体来源
小鼠
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抗体亚型
小鼠IgG1
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抗体描述
Hexokinase I (7A7) Mouse monoclonal antibody
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抗体应用
WB, IHC-P, ICC/IF
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应用推荐
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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特异性
Human, Rat, Mouse
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制备方法
Antigen: Purified recombinant fragment of human HK1 expressed in E. Coli.
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组分
Ascitic fluid containing 0.03% sodium azide.
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储存方法
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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背景介绍
Swiss-Prot Acc.P19367.The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.