Anti-SIRT1 antibody

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  • 抗体类型:多克隆
  • 抗体来源:
  • 抗体应用:WB, IP
  • 特异性:Human SIRT1

产品详情

  • 产品名称
    Anti-SIRT1 antibody
  • 抗体类型
    多克隆
  • 抗体来源
  • 抗体亚型
    兔IgG
  • 抗体描述
    Rabbit polyclonal to SIRT1
  • 抗体应用
    WB, IP
  • 应用推荐

    WB: 10-20 μg/ml

    IP: 0.5-2 uL/mg of lysate

  • 特异性
    Human SIRT1
  • 蛋白别名
    AA673258, RP11-57G10.3, Sir2, Sir2a, Sir2alpha, SIR2L1, Sirt1, MGC150273, SIR2L1, SIRT1, SIR2L1, Sir2, Sir2a, SIR2L1, AA673258, Sir2alpha
  • 制备方法
    Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human SIRT1, and purified by antigen affinity chromatography.
  • 组分
    0.2 μm filtered solution in PBS
  • 储存方法
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • 背景介绍
    SIRT1 belongs to the sirtuin family. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT1 is included in class I of the sirtuin family. It is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. It deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. SIRT1 also deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. It is involved in HES1- and HEY2-mediated transcriptional repression. SIRT1 inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. It may serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. It also deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus.
  • 参考文献
    • Sharma A, et al. (2012) Interactomic and pharmacological insights on human Sirt-1. Front Pharmacol. 3-40.
    • Sun C, et al. (2007) SIRT1 improves insulin sensitivity under insulin-resistant conditions by repressing PTP1B. Cell Metab. 6(4):307-19.
    • Rodgers JT, et al. (2005) Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature. 434(7029):113-8.
    • Nemoto S, et al. (2005) SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1{alpha}. J Biol Chem. 280(16):16456-60.
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