- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IP
- 特异性:Human UBE2H
产品详情
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产品名称
Anti-UBE2H antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Rabbit polyclonal to UBE2H
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抗体应用
ELISA, WB, IP
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应用推荐
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human UBE2H. The detection limit for Human UBE2H is < 0.039 ng/well.
IP: 0.5-2 μg/mg of lysate
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特异性
Human UBE2H
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蛋白别名
1500009C23Rik, AI181839, AI326965, AI462483, AW227540, E2-20K, E2-20K, N28148, UBC8, Ubc8, UBCH, UBCH2, GID3, UBC8, UBCH, UBCH2, E2-20K, Gid3, Ubc8, E2-20K, N28148, AI181839, AI326965, AI462483, AW227540, 1500009C23Rik
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制备方法
Produced in rabbits immunized with purified, recombinant Human UBE2H (rh UBE2H; P62256; Met1-Leu183). UBE2H specific IgG was purified by Human UBE2H affinity chromatography.
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组分
0.2 μm filtered solution in PBS
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储存方法
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
背景介绍
UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
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参考文献
- Sjoerd J L van Wijk, et al. (2009) A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol Syst Biol. 5: 317.
- Nandi D, et al. (2006) The ubiquitin-proteasome system. Journal of biosciences. 31 (1): 137-55.