重组人CD1D蛋白

人

人胚胎肾细胞（HEK293）

Met 1 - Ser 301

A DNA sequence encoding the extracellular domain (Met1-Ser301) of human CD1D (P15813) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the CD1D/B2M heterodimer was purified.

(69.4 + 28.4) % as determined by SDS-PAGE

The recombinant heterodimer of human CD1D/B2M comprises 393 (293+100) amino acids and has a calculated molecular mass of 45 (33.3+ 11.7) KDa. The apparent molecular mass of rh CD1D/B2M heterodimer is approximately 43.2 and 12.5 KDa respectively in SDS-PAGE under reducing conditions.

T-CELL SURFACE GLYCOPROTEIN CD1D; CD1D ANTIGEN; CD1.1; MGC34622; HMC CLASS I ANTIGEN-LIKE GLYCOPROTEIN CD1D; CD1D; D POLYPEPTIDE; CD1D1; AI747460; CD1A; R3G1; DIFFERENTIATION ANTIGEN CD1-ALPHA-3; THYMOCYTE ANTIGEN CD1D; LY-38; ANTIGEN-PRESENTING GLYCOPROTEIN CD1D; CD1D MOLECULE; R3

Antigen-presenting glycoprotein CD1d

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 1 (CD1) is a member of CD system. It's a family of glycoproteins expressed on the surface of various human antigen- presenting cells which are implicated in the presentation of lipid antigens to T-cells. Due to the different lipid anchoring, the CD1 family is classified into two groups: group1 (CD1a-c) and group2 (CD1d). CD1d with lipid antigens activate NK T-cells which rapidly produce Th1 and Th2 cytokines after been activated.

Lyophilized from sterile PBS, pH 7.4.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.