重组小鼠MANF蛋白

产品详情

  • 产品名称
    重组小鼠MANF蛋白
  • 蛋白种属
    小鼠
  • 表达宿主
    人胚胎肾细胞(HEK293)
  • Unitprot ID
  • 蛋白序列
    Met 1 - Leu 179
  • 克隆构建
    A DNA sequence encoding the mouse MANF (NP_083379.2) (Met 1-Leu 179) was expressed, with a C-terminal polyhistidine tag.
  • 蛋白纯度
    > 90 % as determined by SDS-PAGE
  • 分子量
    The secreted recombinant mouse MANF comprises 169 amino acids and has a calculated molecular mass of 19.6 kDa. The apparent molecular mass of the recombinant protein is approximately 21 kDa in SDS-PAGE under reducing conditions.
  • 蛋白别名
    AA673178; MGC142150; MGC142148; AA408789; 3230402M22RIK; D17914; ARP; ARMET; D18MGI17; MANF; AA407711
  • 蛋白全名
    Arginine-rich, mutated in early stage tumors, isoform CRA_b
  • 蛋白描述
    Mesencephalic astrocyte-derived neurotrophic factor, also known as Protein ARMET, Arginine-rich protein, MANF and ARMET, is a secreted protein which belongs to the ARMET family. ARMET selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. ARMET enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. ARMET inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types.
  • 发货形式
    Lyophilized from sterile PBS, pH 7.4
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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