重组小鼠CNDP1蛋白

小鼠

人胚胎肾细胞（HEK293）

Met 1 - Tyr 492

A DNA sequence encoding the mouse CNDP1 (Q8BUG2) (Met 1-Tyr 492) was expressed, with an N-terminal signal peptide and a C-terminal polyhistidine tag.

> 93 % as determined by SDS-PAGE

Measured by its ability to cleave carnosine (ß-Ala-L-His)in a two step assay.
The specific activity is > 250 pmoles/min/μg.

The secreted recombinant mouse CNDP1 comprises 503 amino acids and has a calculated molecular mass of 56.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 55 kDa in SDS-PAGE under reducing conditions.

HST2308; MGC142072; CN1; MGC102737; AI746433; CNDP1; CPGL2; MGC10825

Beta-Ala-His dipeptidase

CNDP1, also known as carnosine dipeptidase 1, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), is a member of the M20 metalloprotease family. The CNDP1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region, which has been demonstrated to be associated with susceptibility to developing diabetic nephropathy, for carnosine protection against the adverse effects of high glucose levels on renal cells. In humans, CNDP1 is secreted from the liver into the serum. In other mammals, including rodents, CNDP1 is expressed exclusively within the kidney and lacks a signal peptide. CNDP1 protein is a secreted homodimeric dipeptidase that specifically hydrolyzes L-carnosine (β-alanyl-L-histidine), and is identified as human carnosinase expressed in the brain. CNDP1 has been associated with diabetic nephropathy in Europeans and European Americans, but not African-Americans. It was identified and confirmed as a risk factor, were cross-sectional and mostly in patients with type 2 diabetes. The polymorphisms of CNDP1 can be excluded as a risk factor for nephropathy in type 1 diabetes. In addition, CNDP1 is also suggested to be implicated in the actions of neuroprotection and neurotransmiting.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.