重组小鼠BACE2蛋白

小鼠

人胚胎肾细胞（HEK293）

Met 1 - Pro 462

A DNA sequence encoding the mouse BACE2 (Q9JL18) (Met1-Pro462) was expressed with a C-terminal polyhistidine tag.

> 95 % as determined by SDS-PAGE

Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010).
The specific activity is >50 pmoles/min/μg.

The recombinant mouse BACE2 comprises 454 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.

ASP21; BETA-SITE AMYLOID BETA A4 PRECURSOR PROTEIN-CLEAVING ENZYME 2; ASPARTIC-LIKE PROTEASE 56 KDA; ASP 1; ALP56; DRAP; DOWN SYNDROME REGION ASPARTIC PROTEASE; ASPARTYL PROTEASE 1; TRANSMEMBRANE ASPARTIC PROTEINASE ASP1; MEMAPSIN-1; ARP1; BAE2; BETA SECRETASE 2; MEMBRANE-ASSOCIATED ASPARTIC PROTEASE 1; 1110059C24RIK; DOWN REGION ASPARTIC PROTEASE; AI850424; BETA-SECRETASE 2; BETA-SITE AMYLOID PRECURSOR PROTEIN CLEAVING ENZYME 2; 56 KDA ASPARTIC-LIKE PROTEASE; ASP1; CDA13; THETA-SECRETASE; BETA-SITE APP CLEAVING ENZYME 2; AEPLC; CEAP1

Beta-secretase 2

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672 (By similarity).

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.