- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC, FC, WB
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
CHOP antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
CHOP Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human breast cancer tissue. Positive WB detected in RAW264.7 cells, A375 cells, HeLa cells, MCF7 cells, Tunicamycin treated HeLa cells. Observed molecular weight by Western-blot: 30 kDa
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抗体应用
ELISA, IHC, FC, WB
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
CEBPZ, CHOP, CHOP 10, DDIT 3, DDIT3, GADD153
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制备方法
This antibody was obtained by immunization of CHOP recombinant protein (Accession Number: NM_004083). Purification method: Antigen affinity purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions to hamster gene.Imposed by unfolded and malfolded proteins, CHOP is significantly induced by ER stress, deficiency of CHOP prevents cell from ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP by forming a heterodimer. It may play an important role in the malignant transformation of nevus to melanoma. The observed molecular weight of CHOP is about 30 kDa.
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参考文献
- Li K, Zhang L, Xiang X. Arsenic trioxide alleviates airway hyperresponsiveness and promotes apoptosis of CD4+ T lymphocytes: evidence for involvement of the ER stress-CHOP pathway. Irish journal of medical science. 182(4):573-83. 2013.
- Sun L, Liu N, Liu SS. Beclin-1-independent autophagy mediates programmed cancer cell death through interplays with endoplasmic reticulum and/or mitochondria in colbat chloride-induced hypoxia. American journal of cancer research. 5(9):2626-42. 2015.
- Huang M, Xu A, Wu X. Japanese encephalitis virus induces apoptosis by the IRE1/JNK pathway of ER stress response in BHK-21 cells. Archives of virology. 161(3):699-703. 2016.
- Lei X, Cui K, Liu Q. Exogenous Estradiol Benzoate Induces Spermatogenesis Disorder through Influencing Apoptosis and Oestrogen Receptor Signalling Pathway. Reproduction in domestic animals = Zuchthygiene. 51(1):75-84. 2016.
- Li J, Liu K, Liu Y. Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. Nature immunology. 14(8):793-803. 2013.
- Xue J, Wei J, Dong X. ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway. The journal of physiological sciences : JPS. 63(6):435-44. 2013.
- Wang SQ, Niu XL, Liu ZW, Zhu YH, Gao DF. Selenium deficiency is associated with endoplasmic reticulum stress in a rat model of cardiac malfunction. Biological trace element research. 156(1-3):196-201. 2013.
- Liu ZW, Zhu HT, Chen KL. Protein kinase RNA-like endoplasmic reticulum kinase (PERK) signaling pathway plays a major role in reactive oxygen species (ROS)-mediated endoplasmic reticulum stress-induced apoptosis in diabetic cardiomyopathy. Cardiovascular diabetology. 12:158. 2013.