- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC, IF, WB
- 特异性:Human; other species not tested.
产品详情
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产品名称
CYP17A1 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
CYP17A1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, HepG2 cells, Jurkat cells, mouse brain tissue, rat brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 48-51 kDa
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抗体应用
ELISA, IHC, IF, WB
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应用推荐
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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特异性
Human; other species not tested.
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蛋白别名
CPT7, CYP17, CYP17A1, CYPXVII, CytOChrome P450 17A1, CytOChrome P450 C17, CytOChrome P450c17, P450C17, S17AH, Steroid 17 alpha monooxygenase
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制备方法
This antibody was obtained by immunization of CYP17A1 recombinant protein (Accession Number: NM_000102). Purification method: Antigen affinity purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
The human cytochrome P450c17 enzyme is encoded by the CYP17A1 gene. It is the qualitative regulator of steroidogenesis in the human adrenals.In its absence, aldosterone is produced in the human adrenal zona glomerulosa .The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta (PMID;19403566). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Ley- dig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme(PMID:15761033). Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5).
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参考文献
- Chrusciel M, Vuorenoja S, Mohanty B. Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice. Journal of cell science. 126(Pt 8):1845-57. 2013.
- Tong A, Jia A, Yan S, Zhang Y, Xie Y, Liu G. Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile. International journal of clinical and experimental pathology. 7(7):4415-21. 2014.
- Huhtinen K, Saloniemi-Heinonen T, Keski-Rahkonen P. Intra-tissue steroid profiling indicates differential progesterone and testosterone metabolism in the endometrium and endometriosis lesions. The Journal of clinical endocrinology and metabolism. 99(11):E2188-97. 2014.
- Bremmer F, Jarry H, Strauß A, Behnes CL, Trojan L, Thelen P. Increased expression of CYP17A1 indicates an effective targeting of the androgen receptor axis in castration resistant prostate cancer (CRPC). SpringerPlus. 3:574. 2014.
- Robitaille CN, Rivest P, Sanderson JT. Antiandrogenic mechanisms of pesticides in human LNCaP prostate and H295R adrenocortical carcinoma cells. Toxicological sciences : an official journal of the Society of Toxicology. 143(1):126-35. 2015.
- Hakkarainen J, Jokela H, Pakarinen P. Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(9):3806-16. 2015.