- Unitprot ID:P21460
A DNA sequence encoding the mouse CST3 (NP_034106.2) precursor (Met 1-Ala 140) was expressed with a C-terminal polyhistidine tag.
> 95 % as determined by SDS-PAGE
Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC,R&D Systems, Catalog # ES009. The IC50 value is < 10 nM.
The secreted recombinant mouse CST3 consists of 131 amino acids and has a calculated molecular mass of 15 kDa. The recombinant protein migrates as three bands (15, 18 & 21-24 kDa) in SDS-PAGE under reducing conditions.
RP23-250M11.9; MGC117328; CYSC; ARMD11; CST3; CYSTATIN C
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Lyophilized from sterile 20mM HEPES, 150mM NaCl, pH 7.0
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
< 1.0 EU per μg of the protein as determined by the LAL method
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.