DDB2 Polyclonal Antibody

多克隆

兔

兔IgG

Polyclonal antibody to DDB2

WB, IF

WB 1:500 - 1:2000
IF 1:50 - 1:200

Human, Mouse, Rat

DDB2, DDBB, UV-DDB2, XPE, DNA damage-binding protein 2

Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-315 of human DDB2 (NP_000098.1).

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Store at -20℃. Avoid freeze / thaw cycles.

Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.