- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IHC, IF
- 特异性:Human, Mouse, Rat; other species not tested.
产品详情
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产品名称
DHX9 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
DHX9 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, mouse spleen tissue, mouse testis tissue, rat spleen tissue, rat testis tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 130 kDa
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抗体应用
ELISA, WB, IHC, IF
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应用推荐
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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特异性
Human, Mouse, Rat; other species not tested.
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蛋白别名
ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA
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制备方法
This antibody was obtained by immunization of DHX9 recombinant protein (Accession Number: BC014246). Purification method: Antigen affinity purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].
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参考文献
- Nowak JS, Choudhury NR, de Lima Alves F, Rappsilber J, Michlewski G. Lin28a regulates neuronal differentiation and controls miR-9 production. Nature communications. 5:3687. 2014.
- Choudhury NR, Nowak JS, Zuo J, Rappsilber J, Spoel SH, Michlewski G. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation. Cell reports. 9(4):1265-72. 2014.