- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IP
- 特异性:Human, Mouse; other species not tested.
产品详情
-
产品名称
EIF4A3 antibody
-
抗体类型
多克隆
-
抗体来源
兔
-
抗体亚型
兔IgG
-
抗体描述
EIF4A3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, human lung tissue, mouse liver tissue, Raji cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 47kd
-
抗体应用
ELISA, WB, IP
-
应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
-
特异性
Human, Mouse; other species not tested.
-
蛋白别名
DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34
-
制备方法
This antibody was obtained by immunization of EIF4A3 recombinant protein (Accession Number: NM_014740). Purification method: Antigen affinity purified.
-
组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
储存方法
Store at -20℃. DO NOT ALIQUOT
-
背景介绍
EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions
-
参考文献
- Yamashita A, Izumi N, Kashima I. SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay. Genes & development. 23(9):1091-105. 2009.
- Izumi N, Yamashita A, Iwamatsu A. AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay. Science signaling. 3(116):ra27. 2010.