- Unitprot ID:P19235
A DNA sequence encoding the extracellular domain (Met 1-Pro 250) of human erythropoietin receptor (NP_000112.1) precursor was fused with a polyhistidine tag at the C-terminus.
> 98 % as determined by SDS-PAGE
Measured by its ability to inhibit EPOdependent proliferation of TF-1 human erythroleukemic cells.
The ED50 for this effect is typically 15-60 ng/mL in the presence of 0.1 U/mL Recombinant Human EPO.
The secreted recombinant human EPOR consists of 237 amino acids and has a predicted molecular mass of 26.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEPOR is approximately 34 kDa due to glycosylation.
EPOR; EPO R; EPO-R; ERYTHROPOIETIN RECEPTOR; EPO RECEPTOR
Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
< 1.0 EU per μg of the protein as determined by the LAL method
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.