重组RSV RSV-F蛋白

RSV

杆状病毒 - 昆虫细胞

Met 1 - Thr 529

A DNA sequence encoding the extracellular domain of human RSV (strain RSS-2) fusion protein (P11209) (Met 1-Thr 529) was expressed with a polyhistidine tag at the C-terminus.

> 95 % as determined by SDS-PAGE

The secreted recombinant human RSV (strain RSS-2) comprises 519 amino acids with a predicted molecular mass of 57.8 kDa. The RSV F0 precursor protein is cleaved into the disulfide-linked F1 and F2 subunits. As a result of glycosylation, the apparent molecular mass of the protein is approximately 63 kDa and 44-53 KDa in SDS-PAGE under reducing conditions, corresponding to the two subunits respectively.

F; PROTEIN F; FUSION PROTEIN (F); RESPIRATORY SYNCYTIAL VIRUS; RSVGP08

Fusion glycoprotein F0

During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2. Interacts directly with heparan sulfate and may participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.