- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IHC, IP, IF, FC
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
GABARAPL1-Specific antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
GABARAPL1-Specific Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in rat brain tissue, HepG2 cells, human heart tissue, mouse liver tissue. Positive IF detected in Chloroquine treated HepG2 cells. Positive IHC detected in human ovary tumor tissue, human liver tissue. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 16 kDa
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抗体应用
ELISA, WB, IHC, IP, IF, FC
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1
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制备方法
This antibody was obtained by immunization of GABARAPL1-Specific recombinant protein (Accession Number: NM_031412). Purification method: Antigen affinity purified.
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组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It’s also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine.The antibody is specific to GABARAPL1, and it doesn’t recognize the recombinant GABARAP and GABARAPL2.
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参考文献
- Boyer-Guittaut M, Poillet L, Liang Q. The role of GABARAPL1/GEC1 in autophagic flux and mitochondrial quality control in MDA-MB-436 breast cancer cells. Autophagy. 10(6):986-1003. 2014.
- Gauthier T, Claude-Taupin A, Delage-Mourroux R, Boyer-Guittaut M, Hervouet E. Proximity Ligation In situ Assay is a Powerful Tool to Monitor Specific ATG Protein Interactions following Autophagy Induction. PloS one. 10(6):e0128701. 2015.
- Keulers TG, Schaaf MB, Peeters HJ. GABARAPL1 is required for increased EGFR membrane expression during hypoxia. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology. 116(3):417-22. 2015.
- Zhao J, Brault JJ, Schild A. FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells. Cell metabolism. 6(6):472-83. 2007.
- Sengupta A, Molkentin JD, Yutzey KE. FoxO transcription factors promote autophagy in cardiomyocytes. The Journal of biological chemistry. 284(41):28319-31. 2009.
- Kassiotis C, Ballal K, Wellnitz K. Markers of autophagy are downregulated in failing human heart after mechanical unloading. Circulation. 120(11 Suppl):S191-7. 2009.
- Mariño G, Fernández AF, Cabrera S. Autophagy is essential for mouse sense of balance. The Journal of clinical investigation. 120(7):2331-44. 2010.
- Jiang S, Heller B, Tagliabracci VS. Starch binding domain-containing protein 1/genethonin 1 is a novel participant in glycogen metabolism. The Journal of biological chemistry. 285(45):34960-71. 2010.