- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC, IP, WB, FC
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
Gamma cystathionase antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Gamma cystathionase Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in mouse liver tissue, HepG2 cells, mouse heart tissue, rat heart tissue, rat liver tissue. Positive FC detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human kidney tissue. Observed molecular weight by Western-blot: 43-45 kDa,66-70 kDa
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抗体应用
ELISA, IHC, IP, WB, FC
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
CSE, CTH, Cystathionine gamma lyase, Gamma cystathionase
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制备方法
This antibody was obtained by immunization of Gamma cystathionase recombinant protein (Accession Number: NM_001902). Purification method: Antigen affinity purified.
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组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation.It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.
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参考文献
- Altaany Z, Yang G, Wang R. Crosstalk between hydrogen sulfide and nitric oxide in endothelial cells. Journal of cellular and molecular medicine. 17(7):879-88. 2013.
- Pan Y, Zhou C, Yuan D, Zhang J, Shao C. Radiation Exposure Promotes Hepatocarcinoma Cell Invasion through Epithelial Mesenchymal Transition Mediated by H2S/CSE Pathway. Radiation research. 185(1):96-105. 2016.
- Flannigan KL, Agbor TA, Motta JP. Proresolution effects of hydrogen sulfide during colitis are mediated through hypoxia-inducible factor-1α. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(4):1591-602. 2015.
- Zhao K, Ju Y, Li S, Altaany Z, Wang R, Yang G. S-sulfhydration of MEK1 leads to PARP-1 activation and DNA damage repair. EMBO reports. 15(7):792-800. 2014.
- Altaany Z, Ju Y, Yang G, Wang R. The coordination of S-sulfhydration, S-nitrosylation, and phosphorylation of endothelial nitric oxide synthase by hydrogen sulfide. Science signaling. 7(342):ra87. 2014.
- Salloum FN, Sturz GR, Yin C. Beetroot juice reduces infarct size and improves cardiac function following ischemia-reperfusion injury: Possible involvement of endogenous H2S. Experimental biology and medicine (Maywood, N.J.). 240(5):669-81. 2015.
- Bos EM, Wang R, Snijder PM. Cystathionine γ-lyase protects against renal ischemia/reperfusion by modulating oxidative stress. Journal of the American Society of Nephrology : JASN. 24(5):759-70. 2013.
- Asimakopoulou A, Panopoulos P, Chasapis CT. Selectivity of commonly used pharmacological inhibitors for cystathionine β synthase (CBS) and cystathionine γ lyase (CSE). British journal of pharmacology. 169(4):922-32. 2013.