重组H1N9 HA蛋白

H1N9

杆状病毒 - 昆虫细胞

Met 1 - Gln 529

A DNA sequence encoding the Influenza A virus(A/mallard/Ohio/265/1987 (H1N9)) hemagglutinin (ABK40634.1)(Met1-Gln529), termed as HA, was expressed with a polyhistidine tag at the C-terminus.

> 90 % as determined by SDS-PAGE.

Measured by its ability to agglutinate guinea pig red blood cells.
HA titer is 0.2-1μg/mL for 1%GRBC

The recombinant hemagglutinin of Influenza A virus (A/mallard/Ohio/265/1987 (H1N9)) consists 523 amino acids and predicts a molecular mass of 58.6 kDa.

C-H-RAS; SPH; C-HA-RAS1; SPNA1; RASH1; CTLO; HA; AF093576; HRAS1; SPNA-1; C-BAS/HAS; NMF4; AI451697; IHJ; P21RAS; H-RASIDX; HAMSV

Hemagglutinin

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.5.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg protein as determined by the LAL method.

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.