重组人ALDH4A1蛋白

人

杆状病毒 - 昆虫细胞

Lys 25 - Gln 563

A DNA sequence encoding the mature form of human ALDH4A1 (AAH07581.1) (Lys 25-Gln 563) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

> 96 % as determined by SDS-PAGE

The recombinant human ALDH4A1/GST chimera consists of 776 amino acids and has a calculated molecular mass of 86.8 KDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.

ALDEHYDE DEHYDROGENASE FAMILY 4 MEMBER A1; DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE; MITOCHONDRIAL; MITOCHONDRIAL DELTA-1-PYRROLINE 5-CARBOXYLATE DEHYDROGENASE; P5CD; AHD-1; P5CDHS; RP23-334I5.1; P5C DEHYDROGENASE; SSDH1; A930035F14RIK; ALDH4; P5CDH; RP11-128M10.1; AHD1; E330022C09; P5CDHL; ALDH5A1

Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.