重组人CST7蛋白

人

人胚胎肾细胞（HEK293）

Met 1 - His 145

A DNA sequence encoding the human Cystatin F precursor (NP_003641.3) (Met 1-His 145) was expressed?with a polyhistidine tag at the C-terminus.

> 92 % as determined by SDS-PAGE

Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The IC50 value is <6 nM.

Cystatin F exists as a disulfide-linked homodimer. The human cystatin F monomer consists of 137 amino acids and and has a predicted molecular mass of 16.1 kDa. As a result of glycosylation, the recombinant Cystatin F migrates as an approximately 44 kDa band in SDS-PAGE under non-reduced conditions corresponding to the dimmer.

RP23-156P22.1; MGC151426; MGC151424; CST7; CMAP; CYSTATIN F

Cystatin-F

The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.