重组人PIP4K2A蛋白

人

人胚胎肾细胞（HEK293）

A DNA sequence encoding the Human PIP4K2A/PIP5K2/PIP5K2A Met1-Thr406 is expressed with a 6His tag at the C-terminus.

>95% as determined by SDS-PAGE.

PIP5K2; PIP5K2A

phosphatidylinositol-5-phosphate 4-kinase type 2 alpha(PIP4K2A)

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. It contains 1 PIPK domain and is expressed ubiquitously, with high levels in the brain. It catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). It may exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. It may regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation, negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P. It also involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.

Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

<1.0 EU per μg protein as determined by the LAL method.

Lyophilized protein should be stored at <-20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days.